Recombinant Human TNF-α/TNFSF2, His #abs00848

abs00848-10ug

1-2 Weeks

138

abs00848-50ug

1-2 Weeks

344

abs00848-1mg

1-2 Weeks

2302

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.0 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

GLYLIYSQVL AHVVANPQAE SSRTPSDKPV NALLANGVEL RDNQLVVPSE LSAIKSPCQR FKGQGCPSTH VLLTHTISRI AVSYQTKVNL GQLQWLNRRA AESGQVYFG IIAL MHHHHHHVRS PEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD IIAL.Please rearrange the above text to create a newly generated content with high similarity to the original text while ensuring that the information from the original information is retained.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the Recombinant HumanTNF-α/TNFSF2, His contains less than 1 EU/μg. Please provide a highly similar revision of the content, using the original information.

Activity

The ED showcases its full biological activity compared to the standard. This indicates that it is just as effective in performing its intended functions.₅₀Based on the original cytotoxicity assay using murine L929 cells, it has been determined that the concentration is below 0.05 ng/ml. This directly translates to a specific activity exceeding 2.0 × 10. Therefore, the data suggests that the substance being tested exhibits a highly potent cytotoxic effect on the L929 cells.⁷ IU/mg in the presence of actinomycin D.

Reconstitution

Before opening this vial, it is recommended to briefly centrifuge it in order to settle the contents at the bottom. To prepare, reconstitute the vial's contents in sterile distilled water or an aqueous buffer that contains 0.1% BSA, aiming for a concentration between 0.1 and 1.0 mg/mL. For long-term storage, divide the stock solution into smaller working aliquots and store them at or below -20 °C. Any further dilutions should be made using appropriate buffered solutions.

Stability & Storage

For long term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated state. However, it can also be stored at 2-8 °C and remains stable under these conditions. Upon reconstitution, the preparation can be stored at 2-8 °C for up to one week. To ensure the utmost stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may compromise its stability.

Target

Accession

P01375

Gene IDs

7124

References

References

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.

2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.

3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.

4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.