Recombinant Human TNF-α/TNFSF2, Variant #abs00849

abs00849-10ug

1-2 Weeks

138

abs00849-50ug

1-2 Weeks

344

abs00849-1mg

1-2 Weeks

2091

Species

Human

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm sieve and subsequently lyophilized. The solution was prepared in PBS and had a pH of 7.0. Can you provide a content that mirrors the original text but uses different words?

Properties

aa_sequence

HQLLNRRANAL MRKRKPVAHV VANPQAEGQL QWLNRRANAL LANGVELRDN QLVVPSEGLY LIYSQVLFKG QGCPSTHVLL THTISRIAVS YQTKVNLLSA IKSPCQRETP EGAEAKPWYE PIYLGGVFQL EKGDRLSAEI NRPDYLDFAE SGQVYFGIIA L

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The amount of Recombinant Human TNF-α/TNFSF2, Variant present in the sample is found to be below 1 EU/μg using the LAL method. In order to create an alternative version of the content, we will rearrange the information while maintaining its consistency.

Activity

The effective dose (ED) of the standard drug was found to be fully biologically active.₅₀The specific activity of less than 0.01 ng/ml, as determined by a cytotoxicity assay using murine L929 cells, indicates a highly potent substance. This potency is reflected by a specific activity greater than 1.0 × 10, demonstrating its efficacy in inducing cytotoxic effects. The novel content is based on the original text but presented in a different manner.⁸ IU/mg in the presence of actinomycin D.

Reconstitution

Before opening the vial, it is recommended to briefly centrifuge it to ensure that the contents settle at the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA, aiming for a concentration of 0.1-1.0 mg/mL. Divide the stock solutions into smaller working aliquots and freeze them at temperatures ≤ -20 °C. When making further dilutions, use suitable buffered solutions.

Stability & Storage

For long-term storage, it is recommended to keep this lyophilized preparation at -20 °C, preferably in a desiccated state. However, it can also remain stable at 2-8 °C. Once reconstituted, it can be stored for up to one week at 2-8 °C. To maintain its stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles in order to preserve its integrity.

Target

Accession

P01375

Gene IDs

7124

References

References

1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.

2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.

3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.

4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.