Recombinant Human IL-21 #abs00826

abs00826-2ug

1-2 Weeks

138

abs00826-10ug

1-2 Weeks

344

abs00826-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered with a 0.2 μm filter and then lyophilized. To create a similar content based on this information, one could say that the PBS solution with a pH of 7.4 was concentrated and then filtered through a 0.2 μm filter before being lyophilized.

Properties

aa_sequence

SMLCEHCHLV QQLVDQLDVN KDVNDLVNYE EFLPAPEDVT WCESAFSCFN SANNQQKKAL SRRIVNEKNR PSKPPKKLRI CSYCDSPTCQ QKKFLERPKP ILQHMKLSLQ GKHHSGHSTS ED.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the Recombinant Human IL-21 contains less than 1 EU/μg. This information can be used to confirm the high quality of the product. It is essential to ensure that the content is highly similar to the original text so that accurate information is provided.

Activity

This product is comparable to the standard in terms of its full biological activity. The effective dose (ED) is the same as that of the standard. To rephrase the information, the product exhibits the same biological activity as the standard, making it fully capable of producing the desired effect. The effective dose required to achieve the desired effect is also equivalent to that of the standard.₅₀Based on the original text information, the specific activity of less than 20 ng/ml, as determined by a cell proliferation assay using human N1186 T cells, exceeds 5.0 × 10.⁴ IU/mg.

Reconstitution

To ensure optimal results, we advise centrifuging the vial briefly before opening it, allowing the contents to settle at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA at a concentration of 0.1-1.0 mg/mL. It is recommended to divide stock solutions into smaller working aliquots and store them at temperatures at or below -20 °C. When making further dilutions, use suitable buffered solutions.

Stability & Storage

To ensure its stability, this lyophilized preparation should be stored at 2-8 °C, although for long term storage it is recommended to keep it at -20 °C in a desiccated state. Upon reconstitution, it can remain stable for up to one week when stored at 2-8 °C. For maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

Q9HBE4

Gene IDs

59067

References

References

1. Pistoia VandCocco C. 2009. J Leukoc Biol, 85: 739-43.

2. Ertelt JM, Johanns TM, Rowe JH, et al. 2010. Immunology, 131: 183-91.

3. Denman CJ, Senyukov VV, Somanchi SS, et al. 2012. PLoS One, 7: e30264.

4. Spolski R, Wang L, Wan CK, et al. 2012. J Immunol, 188: 1924-32.