Recombinant Human IL-22 #abs00827

abs00827-2ug

1-2 Weeks

138

abs00827-10ug

1-2 Weeks

344

abs00827-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 5.0 was filtered through a 0.2 μm filter and then lyophilized. To create a similar content, the information from the original text can be rearranged to express the same message.

Properties

aa_sequence

KLQEINFYPS VVRLNAMQPF FPLDDVNCLR HQKIQQDDSN TGEKLIFGEE LSFHMYCEMS QRLTVNKQFE DLVPEFVLQY RSLRLSNATL DHQIHDLDGE IKGESEGLLK FMFLLDEAGI CNCRLRVSME TKMFALTNRF NPYQQFSNKS CRHMGLEFTI VKNRKDTLGL.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has confirmed that the Recombinant HumanIL-22 contains less than 1 EU/μg. This information suggests that the product is of high quality and meets the necessary standards for use.

Activity

Completely equivalent in terms of biological activity as compared to the standard. The effective dosage is also the same.₅₀The specific activity of inducing IL-10 secretion in human COLO 205 cells is greater than 3.3 × 10, as the corresponding IL-10 secretion level is less than 0.3 ng/ml.⁶ IU/mg.

Reconstitution

It is recommended that the vial be given a brief centrifuge before opening to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA to reach a concentration of 0.1-1.0 mg/mL. Stock solutions must be divided into working aliquots and stored at -20°C or below. Further dilutions can be made using suitable buffered solutions. Please note these instructions carefully to ensure optimal use of the product.

Stability & Storage

To maintain the stability of this lyophilized preparation, it should be stored at temperatures between 2-8 °C. However, for long term storage, it is recommended to keep it at a temperature of -20 °C while ensuring that it is desiccated. Once reconstituted, the preparation can be kept for up to one week at temperatures between 2-8 °C. It is advisable to divide the reconstituted preparation into working aliquots and store them at temperatures between -20 °C to -70 °C for maximum stability. To avoid degradation, it is important to avoid repetitive freeze/thaw cycles.

Target

Accession

Q9GZX6

Gene IDs

50616

References

References

1. Zeng G, Chen CY, Huang D, et al. 2011. J Immunol, 187: 190-9.

2. Cocco CandAiroldi I. 2012. Immunotherapy, 4: 668.

3. Honda K. 2012. Immunity, 37: 952-4.

4. Gurney AL. 2004. Int Immunopharmacol, 4: 669-77.