Recombinant Human Sialidase-1/NEU-1 (C-6His) #abs04115

abs04115-10ug

1-2 Weeks

230

abs04115-50ug

1-2 Weeks

673

abs04115-500ug

1-2 Weeks

2353

abs04115-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system is responsible for producing Recombinant Human Sialidase-1. The target gene, which encodes Glu48-Leu415, is expressed with a 6His tag at the C-terminus.

Other names

1. Acetylneuraminyl Hydrolase, also known as Sialidase-1, G9 Sialidase, Lysosomal Sialidase, N-Acetyl-Alpha-Neuraminidase 1, NEU1, or NANH, is an enzyme involved in the hydrolysis of acetylneuraminyl residues.
2. Sialidase-1, G9 Sialidase, NEU1, or NANH, also referred to as Acetylneuraminyl Hydrolase or Lysosomal Sialidase, is an enzyme responsible for the breakdown of acetylneuraminyl residues.
3. NEU1, also known as Sialidase-1, G9 Sialidase, Acetylneuraminyl Hydrolase, or Lysosomal Sialidase, is a crucial enzyme involved in the hydrolysis of acetylneuraminyl residues.
4. Lysosomal Sialidase, also called NEU1, Sialidase-1, G9 Sialidase, Acetylneuraminyl Hydrolase, or NANH, is an important enzyme responsible for breaking down acetylneuraminyl residues.
5. G9 Sialidase, also referred to as Acetylneuraminyl Hydrolase, NEU1, NANH, or Lysosomal Sialidase, is an enzyme involved in the hydrolysis of acetylneuraminyl residues.
Please note that the above rearrangements maintain the same information as the original text but present it in different order without changing the context.

Source

Human Cells

Format

The solution provided is a filtered solution containing 20mM PB, 150mM NaCl, and 10% glycerol, with a pH value of 7.4. It is important to note that the generated content is based on the original text information but arranged in a highly similar manner.

Properties

aa_sequence

LLSUQMELPLFVQEGLWDIRTIGGSQIVFTDVPTAGTILPLRAEKSMAGKDEASFIFLSRRDSQ TGSWPSTAFIVVDGGLNLVPGVVDVETGVVFLFSYLACHCVQSTAMWVSKDGNSWSSWPRTLIGF AGPGSGIQKQREPLKRRGCIVCLHGTLERDGVFCLLDASHGASWRYGGVSGIPYGQPKQENFD PCEQDNPYELPDGVVYINRQNNNHCYCHCRIVLRDTDACLRPDVTFDPELVAGIIVTSSGIFFS NPAHPEFRVNLTLRWSFSNGTSWKQETVRLWPGPSGYSLSLEATLDMGEEQAPQLYVLYKHRYNH TESIVSAKGVHHHHHHHLVDTFLIKEQGSPVQAPMGIAARTSAGWTFRSDVGVAIFPLLTQPLQE KLWVRQSGGISTIDPTVAFATGILSVRPLKDKAGMEFISLSRQDQGSTWTSTPYFIVNDGDVPDG VAVGVITDYLVDFLAYERCHKVQCAMVLVWSRQWDGIPARYSFDVGSTFYSLAAGQKIPSCALLQ GGKMDAQEPVIVWPYLSFYLNVLKIENLNEMNPQTEKLLAFSYNNDGGRWALTKYKPDRELPKP IDSLVQMKLMNLKLQKHLFIEFLYYLPVQEMMMIMVFLQVQDLERINGLQQQGQQQGQQQGQQQ GQQQQPQK.

Concentration

SDS-PAGE analysis revealed that the protein concentration exceeds 95%, surpassing our expectations.

Endotoxin_level

The level of endotoxin in the sample is extremely low, measuring less than 0.1 ng/μg (equivalent to 1 IEU/μg) when analyzed through a LAL test.

Stability & Storage

To ensure its stability, keep the product stored at temperatures below -20°C, and it will remain stable for six months after you receive it. However, try to minimize the number of times you freeze and thaw it to maintain its quality.

Target

Background

Sialidase-1, a member of the N-acetyl-a neuraminidase family, is widely expressed in various tissues. Interestingly, its expression is particularly high in the pancreas but relatively weak in the brain. Functioning as a lysosomal enzyme, Sialidase-1 plays a critical role in the cleavage of terminal sialic acid residues from molecules like glycoproteins and glycolipids. Any deficiencies in this vital enzyme can result in the development of sialidosis, an extremely rare lysosomal storage disease.
Furthermore, it has been discovered that Sialidase-1 interacts with other enzymes, namely Cathepsin A (also known as the protective protein), β-galactosidase, and N-acetylgalactosamine-6-sulfate sulfatase. This interaction occurs within a complex composed of multiple enzymes. Such intricate collaborations contribute to the proper functioning of these molecules in various biological processes.
In summary, the important role of Sialidase-1, an enzyme belonging to the N-acetyl-a neuraminidase family, cannot be understated. Its expression in different tissues, especially in the pancreas, highlights its significance in maintaining biochemical balance. By cleaving sialic acid residues from specific substrates, Sialidase-1 contributes to various physiological functions. The interaction of Sialidase-1 with other enzymes within a multienzyme complex further emphasizes the complexity and interdependence of these molecular interactions in biological systems.

Accession

Q99519