Recombinant Mouse Midkine #abs01030

abs01030-5ug

1-2 Weeks

138

abs01030-20ug

1-2 Weeks

344

abs01030-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 2 × PBS, pH7.4 was filtered with a 0.2 μm filter before being lyophilized. A highly similar content can be generated by rephrasing the above information to state that the solution was first concentrated and then filtered before undergoing lyophilization.

Properties

aa_sequence

CDGSTGTKAR GSECSEWTWG QGTLKKARYN MGFREGTCGA VAKKKEKVKK CNWKKEFGAD AQCQETIRVT TKAKKGKGKD PCTPSSKDCG CKYKFESWGA QTQRVHCKVP KPCTSKTKSK.
The above text can be rearranged to:
The CK motif is found in many proteins that are involved in signal transduction pathways. This motif is thought to play a role in protein-protein interactions. It is commonly found in protein kinases and in proteins involved in the regulation of transcription. The CK motif may also be involved in the regulation of enzyme activity and cellular localization.

Concentration

> 96 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method confirmed that the rMuMidkine had less than 1 EU/μg. Can you please create a similar message using different wording that conveys the same information?

Activity

The standard and the fully biologically active substance were compared. In the chemotaxis bioassay using human neutrophils, the biological activity of the substance was found to be within the concentration range of 10-100 ng/ml. It exhibits comparable activity to the standard and is highly active at a concentration of 10-100 ng/ml.

Reconstitution

Before opening the vial, it is recommended to briefly centrifuge it in order to settle the contents at the bottom. To reconstitute, add sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. Prepare stock solutions in separate aliquots and store them at -20°C or below. Any further dilutions should be performed using suitable buffered solutions.

Stability & Storage

This freeze-dried formulation can be stored at 2-8 °C, but for long-term storage, it is advisable to keep it at -20 °C and preferably dry. After the preparation has been reconstituted, it can remain stable for up to one week if maintained at 2-8 °C. To ensure maximum stability, it is best to divide the reconstituted preparation into several working aliquots and store between -20 °C to -70 °C. Avoid exposing the preparation to repeated freeze/thaw cycles.

Target

Accession

P12025

Gene IDs

17242

References

References

1. Kaname T, Kuwano A, Murano I, et al. 1993. Genomics. 17:514-5.

2. Ikematsu S, Yano A, Aridome K, et al. 2000. Br J Cancer. 83:701-6.

3. Muramatsu T. 2002. J Biochem. 132:359-71.