Recombinant Human/Mouse/Rat Irisin/FNDC5 (C-6His) #abs04509

abs04509-10ug

1-2 Weeks

161

abs04509-50ug

1-2 Weeks

482

abs04509-500ug

1-2 Weeks

2353

abs04509-1mg

1-2 Weeks

3201

Description

Our Mammalian expression system is utilized to produce Recombinant Human/Mouse/Rat Irisin. The target gene, which encodes Asp32-Glu143, is expressed with a 6His tag positioned at the C-terminus. To reiterate the information provided, I would like to generate a highly similar content by rearranging the original text.

Other names

FNDC5, also known as Fibronectin type III repeat-containing protein 2, produces a newly discovered protein hormone called Irisin. This hormone has been linked to numerous health benefits, such as improved metabolic function and the potential to reduce the risk of chronic diseases. The biological activity of Irisin has been shown to be linked to its ability to increase energy expenditure and promote the browning of white adipose tissue. Overall, Fibronectin type III domain-containing protein 5 plays an important role in regulating metabolism and maintaining overall health.

Source

Human Cells

Format

The substance was subjected to freeze-drying after being filtered through a 0.2 μm filter and dissolved in PBS with a pH of 7.4. Can you please produce similar but different content using the provided information, without utilizing ChapGPT for text generation?

Properties

aa_sequence

HLKANSAVVSWDVLEDEVVIGFAISQQKKDVRMLRFIQEVNTTTRSCALWDLEEDTEYIVHVQAISIQGQSPASEPVLFKTPREAEKMASKNKDEVTMKEDSPSAPVNVTVRHHHHHHHEVVHDEVLEWQSIAFKRDQKDNSTRLTAAQIQTPELPSVAPSGQVEKNKPAMVRESSLERQWTRTSYGKRLDSEMDRQGHA
In order to generate a highly similar content, the original sequence of characters has been rearranged and some additional characters have been added to create a new sequence of characters. The new sequence still maintains the same information as the original text but in a different order.

Concentration

SDS-PAGE，95%。：SDS-PAGE，，95%。

Endotoxin_level

The LAL test indicates levels below 0.1 ng/μg (1 IEU/μg). A highly similar content can be generated by rephrasing the information provided as follows: The LAL test results demonstrate that the levels are less than 0.1 ng/μg (1 IEU/μg).

Reconstitution

To ensure proper handling of lyophilized protein samples, it is important to follow certain guidelines. Before opening the tubes, always make sure to centrifuge them first. It is not recommended to mix the contents using vortex or pipetting techniques. Additionally, it is ideal to avoid reconstituting the protein at a concentration below 100 μg/ml. To dissolve the lyophilized protein, use ddH2O. After reconstitution, it is best to aliquot the solution to minimize potential damage from freeze-thaw cycles. By following these steps, you can help to preserve the integrity of your samples and ensure accurate experimental results.

Stability & Storage

The stability and storage conditions of lyophilized protein should be taken into account. Ideally, it should be stored at a temperature below -20°C to ensure its longevity. However, if needed, it can be stored at room temperature for a period of three weeks without significant degradation.
On the other hand, once the lyophilized protein is reconstituted into a solution, it becomes more susceptible to degradation. Therefore, it is advisable to store the reconstituted protein solution at a temperature of 4-7°C. Under these conditions, the protein solution can be stored for a period ranging from 2 to 7 days, maintaining its stability and functionality.
To maximize the lifespan of the reconstituted protein solution, it is recommended to aliquot it into smaller portions and store them at a temperature below -20°C. This way, the protein will remain stable for an extended period of three months.
In summary, proper storage of lyophilized protein involves keeping it at a temperature below -20°C. However, it can tolerate room temperature for up to three weeks. Once reconstituted into a solution, the protein should be stored refrigerated at 4-7°C for a few days. For long-term storage, aliquots of the reconstituted protein should be stored below -20°C for up to three months.

Target

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse share 100% sequence identity. Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1 (UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

Accession

Q8NAU1