Recombinant Human Interleukin-17A/F Heterodimer/IL-17A & IL-17F (C-6His) #abs04628

abs04628-10ug

1-2 Weeks

230

abs04628-50ug

1-2 Weeks

673

abs04628-500ug

1-2 Weeks

2353

abs04628-1mg

1-2 Weeks

3491

Description

Our Mammalian expression system is capable of producing the Recombinant Human IL-17A & IL-17F Heterodimer. The target gene, which encodes Ile20-Ala155 & Arg31-Gln163, is expressed with a 6His tag at the C-terminus. This ensures that the resulting protein has a high affinity for purification and detection purposes. The heterodimeric nature of IL-17A & IL-17F adds to its biological significance, as it has been shown to play a crucial role in inflammation and immune responses. The inclusion of the 6His tag provides an additional means of monitoring the protein's expression and purification processes.

Other names

IL-17A and IL-17F are two distinct proteins involved in the immune system's response to inflammation. In addition to their individual functions, these proteins can form a heterodimer known as IL-17A/F heterodimer. The IL-17A/F heterodimer is a combination of IL-17A and IL-17F proteins and plays a crucial role in various inflammatory and autoimmune diseases.
By rearranging the content, we can generate a similar text as follows:
The combination of IL-17A and IL-17F proteins forms a heterodimer called IL-17A/F heterodimer. Both IL-17A and IL-17F are important components of the immune system and are involved in the response against inflammation. However, when these two proteins come together to form the IL-17A/F heterodimer, their functions become even more significant.
The IL-17A/F heterodimer plays a critical role in various inflammatory and autoimmune diseases. It acts as a potent pro-inflammatory cytokine, contributing to the recruitment and activation of immune cells, such as neutrophils, monocytes, and T cells, at the site of inflammation. This heterodimer also promotes the production of other inflammatory mediators, including cytokines, chemokines, and matrix metalloproteinases.
The IL-17A/F heterodimer is particularly relevant in conditions like psoriasis, rheumatoid arthritis, and inflammatory bowel diseases, where excessive inflammation and immune dysregulation are observed. It promotes tissue inflammation, remodeling, and destruction, exacerbating the disease progression. Therefore, targeting the IL-17A/F heterodimer has emerged as a potential therapeutic strategy for these diseases, leading to the development of drugs that specifically block its activity.
In summary, the IL-17A/F heterodimer, formed by the combination of IL-17A and IL-17F proteins, plays an important role in inflammation and autoimmune diseases. Understanding its functions and developing targeted therapies can help manage and treat various inflammatory conditions more effectively.

Source

Human Cells

Format

The solution was filtered through a 0.2 μm membrane and then lyophilized. This dry powder was formed from a solution containing 20mM PB, 150mM NaCl, and a pH of 7.4. It is important to note that the generated content is based on the original text information but has been rearranged for clarity and coherence.

Properties

aa_sequence

PRKMTSTLPFSPQVSTSKRISSEFAATMTPGKSLVSLLLLLSLEAIVKAGITIPRNPGCPNSE DKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINA AENCVDGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVAMTSTLPFS PQVSTPRSKFKRISSVLSIEFAATMTVKTLHGPAMVKYLLLSILGLAFLSEAAARKIPKVGHTFFQK PESCMMGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINA QGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHRVQVDHHHHHH.
Here's a possible re-arrangement of the original text:
The sequence MTSTLPFSPQVSTPRSKFKRISSEFAATMTPGKSLVSLLLLLSLEAIVKAGITIPRNPGCPNSE is followed by DKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINA and AENCVDGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVAMTSTLPFS. This is then followed by PQVSTPRSKFKRISSVLSIEFAATMTVKTLHGPAMVKYLLLSILGLAFLSEAAARKIPKVGHTFFQK, which is in turn followed by SCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINA. Finally, the sequence QGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHRVQVDHHHHHH concludes the original text.
And here's a slightly modified re-arrangement:
Starting with MTSTLPFSPQVSTPRSKFKRISSEFAATMTPGKSLVSLLLLLSLEAIVKAGITIPRNPGCPNSE, followed by DKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINA and AENCVDGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVAMTSTLPFS, the text continues with PQVSTPRSKFKRISSVLSIEFAATMTVKTLHGPAMVKYLLLSILGLAFLSEAAARKIPKVGHTFFQK, then SCPPVPGGSMKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINA, and finally QGKEDISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHRVQVDHHHHHH.

Concentration

The SDS-PAGE analysis showed that the purity of the sample was more than 95%. To create a similar statement, we can say that based on the SDS-PAGE results, the sample displayed a purity level greater than 95%.

Endotoxin_level

The result of the LAL test indicates that the concentration is below 0.1 ng/μg (1 IEU/μg). To ensure accuracy, the content generated will be rearranged while maintaining the original information.

Activity

The effectiveness of inducing IL-6 secretion in NIH-3T3 mouse embryonic fibroblast cells was measured, and the ED50 for this impact was found to be below 0.5 ng/ml. To put it differently, the ability to stimulate IL-6 release was evaluated by examining its potency on NIH-3T3 cells, where the concentration required to achieve half-maximal response was determined to be less than 0.5 ng/ml.

Reconstitution

Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 4mM Hcl. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Target

Background

The IL-17 family include IL-17A, IL-17B, IL-17C, IL-17D, IL-17E (also called IL-25), and IL-17F. The family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. All members of the IL-17 family have a similar protein structure, with four highly conserved cysteine residues critical to their 3-dimensional shape, yet they have no sequence similarity to any other known cytokines. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes.

Accession

Q16552 & Q96PD4