Recombinant Human Fatty Acid-Binding Protein 4/FABP4/A-FABP/aP2 (N-6His) #abs04306

abs04306-10ug

1-2 Weeks

161

abs04306-50ug

1-2 Weeks

482

abs04306-500ug

1-2 Weeks

2353

abs04306-1mg

1-2 Weeks

3201

Description

Our E.coli expression system has successfully produced Recombinant Human FABP4, a protein encoded by the target gene Cys2-Ala132. At the N-terminus of this protein, a 6His tag has been included for identification purposes. This process has enabled us to create a high-quality product that closely matches the characteristics of FABP4 found in the human body. Our robust production method ensures that the recombinant protein is pure, potent, and biologically active.

Other names

Adipocyte Lipid-Binding Protein, also known as Fatty Acid-Binding Protein Adipocyte, ALBP, Adipocyte-Type Fatty Acid-Binding Protein, A-FABP, AFABP, or Fatty Acid-Binding Protein 4, is a protein that plays a vital role in the metabolism of lipids in adipocytes. Its main function is to facilitate the transport of fatty acids from the cytoplasm to the mitochondrial matrix for beta-oxidation.
This protein is highly expressed in adipocytes and is involved in the regulation of insulin sensitivity and glucose uptake. Studies have shown that increased levels of ALBP are associated with obesity, insulin resistance, and type 2 diabetes.
ALBP has also been found to be involved in lipid metabolism in non-adipose tissues, such as the liver, skeletal muscle, and heart. Its increased expression in these tissues has been linked to the development of metabolic disorders.
In summary, ALBP plays a crucial role in the metabolism of lipids in adipocytes, and its dysregulation has been linked to the development of metabolic disorders such as obesity and diabetes. It is a promising target for the development of therapeutics aimed at treating these conditions.

Source

Escherichia coli.

Format

The solution containing 20mM PB, 150mM NaCl, pH 7.4 was first filtered through a 0.2 μm filter. After filtration, the solution was lyophilized, resulting in a desiccated product. The lyophilized product retains the same composition as the original solution, ensuring the preservation of 20mM PB, 150mM NaCl, and a pH of 7.4. This lyophilized form offers improved stability and long-term storage capabilities compared to the liquid solution. It can be reconstituted with a suitable solvent when needed, without compromising its original composition.

Properties

aa_sequence

GVGFATRKVAGMAKPNMIISVMGSSHHHHHHSSGLVPRGSHMCDAFVGTWKLVSSENFDDYMKEV NGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKRKREDDKL SSENFDDYMKEVGVGFATRKVAGMAKPNMIISV NGDVITIKSESTFKNTEISFILGQEFDEVTADDRKVKSTITLDGGVLVHVQKWDGKSTTIKR It is important to rearrange the content to create a highly similar text. However, the generated content should be based on the original information provided.

Concentration

The determination of greater than 95% through the reduction of SDS-PAGE indicates a high level of similarity in the content. An alternative approach to speaking about the same information could be to express the significance of achieving such a result in protein analysis. SDS-PAGE reduction has confirmed a remarkable purity level exceeding 95%, underscoring the precision and reliability of the experimental method employed.

Endotoxin_level

The LAL test has determined that the level of endotoxin in the substance is less than 0.1 ng/μg (1 IEU/μg). It is important to ensure that such low levels are maintained to ensure the safety and efficacy of the substance.

Reconstitution

It is important to always centrifuge tubes before opening them to ensure proper mixing. Vortexing or pipetting the contents can cause inconsistencies in the resulting solution. Additionally, it is recommended to reconstitute the lyophilized protein to a concentration no less than 100 μg/ml. To dissolve the protein, use ddH2O and ensure to aliquot the solution to avoid excessive freeze-thaw cycles. By following these guidelines, it is possible to generate a highly similar content that is based on the original text information.

Stability & Storage

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target

Background

Fatty Acid-Binding Protein 4 (FABP4) is a cytoplasm protein that belongs to the fatty-acid binding protein (FABP) family of calycin superfamily. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids. FABP4 is expressed in a differentiation-dependent fashion in adipocytes and is a critical gene in the regulation of the biological function of these cells. FABP4 is thought to participate in Lipid transport protein in adipocytes. FABP4 binds to the long chain fatty acids and retinoic acid, delivers long-chain fatty acids and retinoic acid to their cognate receptors in the nucleus. FABP4 modulates inflammatory responses and cholesterol ester accumulation. FABP4 is a plasma marker of metabolic disturbances in HIV-infected patients, and therefore, could serve to guide therapeutic intervention in this group of patients.

Accession

P15090