Recombinant Human PEDF #abs00845

abs00845-5ug

1-2 Weeks

138

abs00845-20ug

1-2 Weeks

344

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl was filtered through a 0.2 μm filter and subsequently lyophilized.

aa_sequence

AGTKLIDKLT GMPALDLVSL LAVAEKPQNL VSLVRDYAFG RNSTSLPTVT LASASTALSG RLTARQESEL IYHRVSILIY GSPDIDTSHL TFIDHSVPLR EYILTSVGDL PYDRGGITKT PAVEKNLQKS SKVIKLFEKR LEFKPVAISS SRPTRTSETL TVDEKNIINW IIQSGKMKAG KELKIEISTD ELLVGAHFQK VKWRGSLDTF TDKYMERVDE KPLMSASLPG IDSYKKTRLN NESTQFILKQ HFTIDHSEES ISILQTVKVA QLKPLPSKSY SKLQETSMEL LFLNPKTSID GPGTKKIILE LKQEHRAFGW NEWTDSLPGA LTPSFHAQLP DLNYLNVFQF LKRDYVLTDI TGFAPYLIIL LIGTLQNVTE EIHSLSTEEF MQLIEKVTVK SLQKNEWPLQ TKILGTGSAP PKLPFGGHAT TPIFTVNIRK.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the Recombinant Human PEDF has been found to contain less than 1 EU/μg. This information is being rearranged in a highly similar format to convey the same message effectively.

Activity

When compared to the standard, the ED is fully biologically active. Please rearrange the above content to generate a highly similar version.₅₀The specific activity of this protein, which is measured by its capacity to boost the adhesion of human Saos2 cells to plate coated with bovine Collagen I, is less than 2 ng/ml. This indicates a rather potent activity of > 5.0 × 10, making it an impressive protein with excellent adhesion properties.⁵ IU/mg.

Reconstitution

To ensure proper mixing of the contents, it is recommended to briefly spin the vial before opening. The protein can be reconstituted by adding sterile distilled water or an aqueous buffer containing 0.1% BSA. The resulting concentration should be within the range of 0.1-1.0 mg/mL. To maintain stability, it is advisable to divide the stock solution into working aliquots and store them at temperatures ≤ -20 °C. Any further dilutions should be carried out in buffered solutions appropriate for the intended use.

Stability & Storage

The lyophilized form of this product can be safely stored at 2-8 °C; however, for long-term storage, it is recommended that it be kept at -20 °C, preferably in a desiccated state. When reconstituted, the preparation can be used for up to a week if stored at 2-8 °C. For optimal stability, it is advisable to divide the reconstituted solution into working aliquots and to store them at -20 °C to -70 °C. Repeated freeze/thaw cycles should be avoided at all costs to maintain the product's efficacy.

P36955

Gene IDs

5176

References

1. Bouck N. 2002. Trends Mol Med, 8: 330-4.
2. Sugita Y, Becerra SP, Chader GJ, et al. 1997. J Neurosci Res, 49: 710-8.
3. Takenaka K, Yamagishi S, Jinnouchi Y, et al. 2005. Life Sci, 77: 3231-41.
4. Yamagishi S, Matsui T, Nakamura K, et al. 2007. Protein Pept Lett, 14: 615-7.
5. Volpert KN, Tombran-Tink J, Barnstable C, et al. 2009. J Ocul Biol Dis Infor, 2: 1-11.