Recombinant 2019-nCoV S Protein RBD-SD1 (C-6His) #abs04708

abs04708-50ug

1-2 Weeks

310

abs04708-500ug

1-2 Weeks

2328

Description

Our Mammalian expression system has produced Recombinant 2019-nCoV S Protein RBD-SD1, where the target gene encoding Arg319-Ser591 is expressed with a 6His tag at the C-terminus. For those seeking highly similar content, rearrangement of the original text is necessary to convey the same message effectively.

Other names

2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein

Source

Human Cells

MW

，：31.4kDa（AP：35-43kDa）。，，ChapGPT。

Format

Supplied as 0.2μM filtered PBS solution, pH 7.4.

Properties

Purification

Affinity purification chromatography.

Concentration

The level of purity was found to be over 95% by analyzing the protein through SDS-PAGE gel electrophoresis. It can be concluded that the protein sample is highly homogeneous and free of contaminants.

Stability & Storage

The reconstituted protein solution must be stored at a temperature not exceeding -20°C for optimal preservation. It is crucial to follow this storage guideline to ensure that the protein solution remains functional and effective for its intended use. Improper storage conditions can significantly impact the stability and performance of the protein, resulting in a loss of activity or function. Therefore, it is recommended to store the reconstituted protein solution in a freezer or equivalent facility.

Target

Background

Protein S (PROS1) is a vital glycoprotein involved in various biological processes, including coagulation, apoptosis, cancer development and progression, and the innate immune response. Its expression in different cell types suggests its crucial role in diverse physiological functions. The receptors that bind S1 include ACE2, angiotensin-converting enzyme 2, DPP4, CEACAM, and others. Coronaviruses use the spike (S) glycoprotein in binding to the host cell during the infection process. The S glycoprotein is pivotal in severe acute respiratory syndrome (SARS) infections caused by severe acute respiratory syndrome-coronavirus (SARS-CoV). It mediates membrane fusion and cell entry and is a major immunogen and entry inhibitor's target. Recent studies have reported that 2019-nCoV targets human respiratory epithelial cells via ACE2.
The S protein of coronaviruses is a large type I transmembrane protein consisting of two subunits, S1 and S2. The RBD domain, located in S1, plays a crucial role in recognizing the cell surface receptor for virus entry, while S2 contains basic elements necessary for membrane fusion. The S protein plays a pivotal role in inducing neutralizing-antibody and T-cell responses and offering protective immunity in coronaviruses. Its significance in pathogenesis makes it a target for potential treatments and vaccines development.