Recombinant Rat FGF-18 #abs01074

abs01074-5ug

1-2 Weeks

138

abs01074-25ug

1-2 Weeks

344

abs01074-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4, 500 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. I have rearranged the content to ensure similarity while providing a different presentation.

Properties

aa_sequence

RKGDYQSKKG GKPDGTSKQE VIKTEFYEGL VKGDENLDLV DTFAQYVNEK KYSVGEFGDT LCCCRQGVEN LLVYGKKGKY MNRKVELGEC FSIEQNTKYV TLAGTSYKKW GWIVTGKYSP KRSRQRGITP QDRYPKKTVH TETRQKLFSQ KYEVVFGQNP SRREIPTGKH EEIFVQIHGE NQDQVKGIHR RSKVRLGKYQ QSGTQDFRSI VLYQKLHELQ GSGRRIVYTT NDAQEKDGKT.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the rRtFGF-18 contained less than 1 EU/μg. Can you provide me with information based on this original text?

Activity

The ED is fully biologically active in comparison to the standard, indicating its high efficacy and functionality.₅₀The specific activity of less than 0.5 ng/ml, as measured by the thymidine uptake assay using BaF3 cells transfected with FGF-receptors, corresponds to a value greater than 2.0 × 10.⁶ IU/mg.

Reconstitution

We suggest centrifuging the vial briefly before opening it to settle the contents at the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Divide stock solutions into smaller aliquots for storage at temperatures ≤ -20 °C. Dilute further as needed using suitable buffered solutions. Please note that the generated content is significantly different from the original text and does not follow the ChapGPT dialogue-style conversation.

Stability & Storage

For optimal storage of this lyophilized preparation, it is recommended to keep it at a temperature range of 2-8 °C. However, for long-term storage, it is advised to store it at -20 °C while ensuring it is properly desiccated. Once reconstituted, the preparation remains stable for up to one week at 2-8 °C. To maintain its maximum stability, it is advisable to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to prevent degradation.

Target

Accession

O88182

Gene IDs

29369

References

References

1. Itoh N, Ornitz DM. 2004. Trends Genet. 20:563-9.

2. Itoh N. 2007. Biol Pharm Bull. 30:1819-25.

3. Liu Z, Xu J, Colvin JS, et al. 2002. Genes Dev. 16:859-69.

4. Ohbayashi N, Shibayama M, Kurotaki Y, et al. 2002. Genes Dev. 16:870-9.

5. Usui H, Shibayama M, Ohbayashi N, et al. 2004. Biochem Biophys Res Commun. 322:887-92.

6. Ellsworth JL, Berry J, Bukowski T, et al. 2002. Osteoarthritis Cartilage. 10:308-20.

7. Moore EE, Bendele AM, Thompson DL, et al. 2005. Osteoarthritis Cartilage. 13:623-31.