Recombinant Rat KGF-2/FGF-10 #abs01073

abs01073-5ug

1-2 Weeks

138

abs01073-25ug

1-2 Weeks

344

abs01073-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5% trehalose was subjected to filtration through a 0.2 μm filter. The resulting solution was then lyophilized to obtain a lyophilized product.

Properties

aa_sequence

AVSSNGKKSE VTTVIEISL SSSSSSSFSS PSSAGRHVRS LKIEKNGKVS YNHLQGDVRW RKLFSFTKYF GTKKENCPYS ILEIQSVEIG GYNTYASFNW YYLAMNKKGK LYGSKEFNND CKLKERIEEN QALGQDMVSP HFLPMVVHS WVNGRQMYAA RHLAGPQSKG QKRRRKNSTG VAVKNINASN
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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has shown that the presence of rRtKGF-2/FGF-10 is less than 1 EU/μg. This information can be rephrased to convey the same meaning as the original text.

Activity

Compared to the standard, the substance is completely biologically active. The effective dosage (ED) of the product is equivalent to that of the standard.₅₀The specific activity of a cell proliferation assay using monkey 4MBr-5 cells was found to be greater than 8.3 × 10 when the determined concentration of the substance is less than 120 ng/ml. This information is important in understanding the potency of the substance and its potential effects on cell growth and proliferation. Further studies may be necessary to determine the exact mechanism of action and potential therapeutic applications of this substance.³ IU/mg.

Reconstitution

We advise a brief centrifugation of the vial prior to opening in order to settle the contents at the bottom. Reconstitute the substance in sterile distilled water or an aqueous buffer containing 0.1% BSA, achieving a concentration of 0.1-1.0 mg/mL. Divide the stock solutions into smaller working aliquots and store them at or below -20 °C. For further dilutions, employ suitable buffered solutions.

Stability & Storage

For optimal preservation, it is recommended to store this lyophilized preparation at -20 °C while ensuring it is desiccated. However, it can be stored at 2-8 °C as well. Once reconstituted, the preparation remains stable for up to one week when refrigerated at 2-8 °C. To maintain its maximal stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles to ensure its integrity.

Target

Accession

P70492

Gene IDs

25443

References

References

1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.

2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.

3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.

4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.