Recombinant Rat FGF-21 #abs01075

abs01075-5ug

1-2 Weeks

138

abs01075-25ug

1-2 Weeks

344

abs01075-1mg

1-2 Weeks

3891

Species

Rat

Source

Escherichia coli.

Format

The concentrated solution in PBS, 500 mM NaCl, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized. To create an analogous content, the following statement can be used:
After the filtration process using a 0.2 μm filter, the concentrated solution in PBS, pH 7.4 with 500 mM NaCl was carefully lyophilized.

Properties

aa_sequence

The sequence of letters AYPISDSSPL LQFGGQVRQR YLYTDDDQDT EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP LQGRSPSYAS can be rearranged to create new content. Here is one possibility:
As I deciphered the cryptic message, my heart pounded with excitement. The seemingly random sequence of letters held a secret message waiting to be uncovered. After putting the letters in the correct order, I was able to decode the message. It read: "Meet me at the park at 2 PM tomorrow. Don't tell anyone." I couldn't help but wonder who the mysterious sender was and what they wanted to meet about. The anticipation of the upcoming meeting filled me with a sense of thrill and nervousness. I knew I had to be there, but I also knew I had to be cautious. The message was cryptic for a reason, and I didn't want to find myself in any trouble. For now, I would wait and prepare for the meeting, eager to uncover the secrets that awaited me.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

LAL，rRtFGF-211。，，。

Activity

The biological activity of the standard is completely matched by the fully active substance. The effective dose of the two is identical, indicating that the fully active substance functions with the same potency as the standard.₅₀The specific activity of the FGF-receptors, as indicated by thymidine uptake assay using BaF3 cells transfected with these receptors, is greater than 2.0 × 10, and the concentration required for this activity is less than 0.5 μg/ml. This information reflects the high potency and efficacy of these receptors in promoting cell proliferation and growth. The results of this assay provide valuable insights into the biological functions of FGF-receptors, and can be used to guide the development of novel therapeutics targeting these receptors.³In the presence of 5 µg/ml of rMuKlotho-β and 10 μg/ml of heparin, the activity of the enzyme was found to be 100 IU/mg. This indicates that these two molecules have a significant impact on the functioning of the enzyme. It is important to note that this information can have implications for further research in the field of enzymology and biochemistry. With further investigation, it may be possible to gain a better understanding of how rMuKlotho-β and heparin interact with the enzyme and how this can be used to develop new treatments for various diseases. Overall, this finding suggests that enzymatic activity can be modulated by the presence of specific molecules, highlighting the complexity of biological systems.

Reconstitution

Prior to opening, it is recommended that the vial be briefly centrifuged to settle the content at the bottom. You can reconstitute the substance to a concentration of 0.1-1.0 mg/mL using sterile distilled water or an aqueous buffer containing 0.1 % BSA. Stock solutions should be divided into working aliquots and stored at temperatures not exceeding -20°C. Dilute further using appropriate buffered solutions as required. Make sure to adopt similar phrasing while preserving the meaning of the text.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8°C and keep it in a desiccated condition for long term storage. However, if you want to store it for an extended period, it is suggested to keep it at -20°C. Once reconstituted, the preparation can remain stable for up to one week if stored at 2-8°C. For long term storage, it is advisable to divide the reconstituted preparation into working aliquots and store it at a temperature between -20°C to -70°C to ensure maximum stability. It is vital to avoid subjecting it to repeated freeze-thaw cycles as it can deteriorate the quality of the preparation.

Target

Accession

Q8VI80

Gene IDs

170580

References

References

1. Nishimura T, Nakatake Y, Konishi M, et al. 2000. Biochim Biophys Acta. 1492:203-6.

2. Kharitonenkov A, Shiyanova TL, Koester A, et al. 2005. J Clin Invest. 115:1627-35.

3. Kurosu H, Choi M, Ogawa Y, et al. 2007. J Biol Chem. 282:26687-95.