Recombinant Mouse GM-CSF #abs00994

abs00994-5ug

1-2 Weeks

138

abs00994-20ug

1-2 Weeks

344

abs00994-1mg

1-2 Weeks

4214

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized into a powder. The original solution was in PBS with a pH of 7.4.

Properties

aa_sequence

STRTACIVPKR EPKDNIAKVL EMVVDPAEEL TKLENEFVVE KTNFSVKLEF FQVTCRILTK LRGKLKGANT LTCNFMRAGT SYPQYCTETP VPNKDTDTCE VYSFAKKFLT KL

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The level of rMuGM-CSF is determined to be lower than 1 EU/μg using the LAL method. To create similar content, the information could be rephrased as follows: The LAL method was used to measure the amount of rMuGM-CSF, which was found to be less than 1 EU/μg.

Activity

The standard and the tested substance have comparable biological activity. The tested substance is fully active, exhibiting no significant differences from the standard.₅₀The specific activity of this protein, as determined by a cell proliferation assay utilizing murine FDC-P1 cells, is greater than 2.0 × 10 and the concentration required to achieve this level of activity is less than 0.05 ng/ml. These findings demonstrate the potent biological activity of this protein, which can play a crucial role in various physiological processes.⁷ IU/mg.

Reconstitution

Before opening the vial, we suggest briefly centrifuging it to settle the contents at the bottom. To reconstitute, you can use sterile distilled water or an aqueous buffer with 0.1% BSA at a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into smaller working aliquots and store them at -20 °C or below. When making further dilutions, make sure to use appropriate buffered solutions.

Stability & Storage

To maintain the stability of the lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it is best to keep it at -20 °C while ensuring that it remains desiccated. Once reconstituted, the preparation can last for up to one week when stored at 2-8 °C. For optimal stability, it is advisable to divide the reconstituted preparation into small aliquots and store them at -20 °C to -70 °C. Avoid exposing the preparation to repeated freeze/thaw cycles, as this may affect its stability.

Target

Accession

P01587

Gene IDs

12981

References

References

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.

2. 1989. N Engl J Med, 320: 253-4.

3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.

4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.

5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.