Recombinant Human M-CSF #abs00846

abs00846-2ug

1-2 Weeks

138

abs00846-10ug

1-2 Weeks

344

abs00846-1mg

1-2 Weeks

5575

Species

Human

Source

Escherichia coli.

Format

The substance was dried through lyophilization after being filtered through a 0.2 μm mesh and dissolved in PBS at pH 7.4. It is important to note that the resulting content is highly similar to the original text and is based on the same factual information.

Properties

aa_sequence

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Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The concentration of Recombinant Human M-CSF is determined to be less than 1 EU/μg using the LAL method. By rearranging the content, the following highly similar content is generated: The LAL method was employed to determine the level of Recombinant Human M-CSF, and it was found to be lower than 1 EU/μg.

Activity

The biological activity of the sample is comparable to that of the standard, indicating that it is fully functional. The effective dose (ED) of the sample has been measured and found to be similar to that of the standard.₅₀Cell proliferation assay using murine M-NFS-60 cells revealed that the concentration of the substance is below 1 ng/ml, demonstrating a specific activity higher than 1.0 × 10.⁶ IU/mg.

Reconstitution

Prior to opening this vial, we suggest briefly centrifuging it to settle the contents to the bottom. Reconstitute the material with sterile distilled water to achieve a concentration of 0.1 mg/mL. For long-term storage, divide the stock solutions into working aliquots and keep them frozen at temperatures of or below -20°C. Appropriate buffered solutions should be used for subsequent dilutions.

Stability & Storage

To maintain the integrity of this lyophilized preparation, it is recommended to store it at a temperature range of 2-8°C and keep it desiccated for long term storage. However, for ultimate preservation, it is advised to store it at a temperature of -20°C. Once reconstituted, the preparation can remain stable for up to one week if kept refrigerated at a temperature range of 2-8°C. To prolong its stability, divide the reconstituted preparation into working aliquots and store them at a temperature range of -20°C to -70°C. To prevent deterioration, avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P09603-3

Gene IDs

1435

References

References

1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.

2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.

3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.

4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.