Recombinant Human G-CSF #abs00838

abs00838-2ug

1-2 Weeks

138

abs00838-10ug

1-2 Weeks

344

abs00838-1mg

1-2 Weeks

6742

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in 10mM sodium acetate buffer, with 5% trehalose and pH level of 4.0, has been filtered through a 0.2 μm filter and lyophilized. This process results in a highly concentrated powder that can be easily stored and transported for further use.

Properties

aa_sequence

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Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method detected Recombinant Human G-CSF at levels lower than 1 EU/μg. Can you please create new content based on this information, but using different wording?

Activity

The standard and the fully biologically active version were compared, and it was found that they have no significant difference. This means that the fully biologically active version is just as effective as the standard one.₅₀The specific activity of this substance was measured using a cell proliferation assay with murine NFS-60 cells, resulting in a value of less than 0.1 ng/ml. This indicates a high potency, with a specific activity greater than 1.0 × 10. It is evident that this substance exhibits strong biological activity and has the potential for various applications. Such information is crucial in the development of drugs and therapeutics, and can contribute to the advancement of medical research.⁷ IU/mg.

Reconstitution

It is recommended to gently centrifuge the vial before opening to settle the contents at the bottom. The protein should be reconstituted using either sterile distilled water or an aqueous buffer with 0.1% BSA to obtain a concentration of 0.1-1.0 mg/mL. It is advised to separate the protein into working aliquots and keep them frozen at ≤ -20 °C. Further dilutions should be performed using buffered solutions appropriate for the intended application.

Stability & Storage

To ensure the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C, with long-term storage at -20 °C, preferably in a desiccated form. Upon reconstitution, the preparation can be kept for up to one week at 2-8 °C. For optimal stability, divide the reconstituted solution into working aliquots and store them at ultra-low temperatures ranging from -20 °C to -70 °C. Avoid frequent freezing and thawing cycles that may affect its efficacy.

Target

Accession

P09919

Gene IDs

1440

References

References

1. Scarffe JHandKamthan A. 1990. Cancer Surv, 9: 115-30.

2. Fattorini L, Xiao Y, Li B, et al. 1994. J Med Microbiol, 40: 129-33.

3. Tsavaris N, Kosmas C, Gouveris P, et al. 2004. Med Sci Monit, 10: PI24-8.

4. Shochat E, Rom-Kedar V, Segel LA. 2007. Bull Math Biol, 69: 2299-338.

5. Cornish AL, Campbell IK, McKenzie BS, et al. 2009. Nat Rev Rheumatol, 5: 554-9.