Recombinant Mouse M-CSF #abs00996

abs00996-2ug

1-2 Weeks

138

abs00996-10ug

1-2 Weeks

344

abs00996-1mg

1-2 Weeks

5575

Species

Murine

Source

Escherichia coli.

Format

The solution was filtered through a 0.2 μm filter and then lyophilized. The lyophilized product was derived from a solution containing 20 mM Tris, 500 mM NaCl, and pH 7.4.

Properties

aa_sequence

LLKKIEMNQH DVLDSSMQCI EFLDQIFVAE PVLRPDDQCE FFLQVDIKAF NFKDMRTEID LPSANQTERL NSNNLSQCEK QEYENKDAFC FEHTFRTVAC IKNFKELLEQ NETKDNELLF TNNKWIFDCN RCSSDAKSFT DCNCPTKPYS SAPSPDPATA ALAWSLMAPP GSETRQDWSL LPRESLPLS SQRKGADSPE LCTQTSPRRP.

Concentration

> 95 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The quantity of rMuM-CSF determined using the LAL method is less than 1 EU/μg. Let me generate a text that maintains the essence of the original information but is phrased differently: The LAL method indicates that the rMuM-CSF level is below 1 EU/μg, demonstrating its minimal presence in the sample.

Activity

Standard comparison reveals full biological activity. The ED is effective. Please produce a substantially similar content by reorganizing the given information while ensuring that the generated content is based on the original text. Do not generate content using the same method as ChapGPT, but instead, deliver a speech in a completely different manner from text-based language models.₅₀The specific activity of the substance, as assessed through a cell proliferation assay involving murine M-NFS-60 cells, is greater than 5.0 × 10, with a corresponding concentration of less than 2 ng/ml. This finding underscores the potent nature of the substance and its ability to stimulate cell growth in a highly effective manner.⁵ IU/mg.

Reconstitution

Before opening, it is advisable to briefly centrifuge the vial in order to bring the contents to the bottom. To prepare, reconstitute the vial in sterile distilled water or an aqueous buffer containing 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. It is recommended to divide the stock solutions into smaller working aliquots and store them at a temperature of -20 °C or below. For further dilutions, utilize appropriate buffered solutions.

Stability & Storage

To maintain the integrity of this lyophilized preparation, it is crucial to store it properly. The preparation can be kept stable at 2-8 °C, but for long-term storage, it is recommended to store it at -20 °C while keeping it desiccated. After reconstitution, the preparation can remain stable for up to one week at 2-8 °C. However, for optimal stability, the reconstituted preparation should be divided into appropriate working aliquots and preserved at a temperature ranging from -20 °C to -70 °C. Be mindful to avoid repeated freeze-thaw cycles as it may affect the composition of the lyophilized preparation.

Target

Accession

Q3U4F9

References

References

1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26.

2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52.

3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35.

4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.