Recombinant Rhesus Macaque GM-CSF #abs00962

abs00962-2ug

1-2 Weeks

138

abs00962-10ug

1-2 Weeks

344

abs00962-1mg

1-2 Weeks

6742

Species

Rhesus Macaque

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm membrane and then lyophilized. To provide a similar content based on the original text information, we could say that the solution was processed for preservation by removing water through a lyophilization process after being concentrated and filtered for purity. The pH level was maintained within the optimal range of 7.4 by dissolving the solution in PBS before filtration and lyophilization.

Properties

aa_sequence

EPASNARPSPGT AQWEIHVNAIQ EALLRRLNSR DTAAEMNKTVE EVVSEMFDLQ EQPSCRLTLE YKQLGGLQGS LTKLPGTMMH AKYQHQCPP TPETSCATQI ITFQSFKLNE KDLFLIVPFD CWEPVQE.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the content states that the rRhGM-CSF has a concentration of less than 1 EU/μg. To generate highly similar content by rearranging the above information, we can say: "The determined concentration of rRhGM-CSF, using the LAL method, is less than 1 EU/μg."

Activity

The biological activity of the sample is equivalent to the standard, indicating that it is fully functional. This pertains to the ED. To create similar content while retaining the essence of the original statement, one could say that the sample displays comparable biological activity to the standard and therefore exhibits full functionality with respect to the ED.₅₀The cell proliferation assay conducted with human TF-1 cells has shown that the amount of the protein in question is less than 0.1 ng/ml. This indicates a specific activity level of greater than 1.0 × 10, demonstrating the potent biological effects of the protein. The data suggests the potential for exciting new directions in medical research and therapy development, and highlights the importance of continued investigation into the properties and behavior of this important protein.⁷ IU/mg.

Reconstitution

We suggest performing a brief centrifugation of the vial before opening to ensure the contents settle at the bottom. Reconstitution should be done using sterile distilled water or an aqueous buffer which consists of 0.1% BSA to attain a concentration of 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into working aliquots and store them at temperatures of no more than -20 °C. Any further dilutions should be carried out using the appropriate buffered solutions.

Stability & Storage

For optimum storage conditions, this lyophilized preparation should be kept at -20 °C, preferably in a desiccated state. However, it can also remain stable at 2-8 °C. When reconstituted, it can remain stable for up to one week if stored at 2-8 °C. To ensure maximum stability, it is recommended to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is important to avoid subjecting the preparation to repeated freeze/thaw cycles as it may impact its stability.

Target

Accession

Q9GL44

References

References

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.

2. 1989. N Engl J Med, 320: 253-4.

3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.

4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.

5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.