Recombinant Human GM-CSF #abs00839

abs00839-5ug

In Stock

138

abs00839-20ug

In Stock

344

abs00839-1mg

In Stock

4214

Species

Human

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized.

Properties

aa_sequence

IPTFSSFENL EPTCLQTRLE LYKQGLRGSL ASHYKQHCPP DTAAEMNETV APARSPSPST EVISEMFDLQ QPWEHVNAIQ TKLKGPLTMM EARRLLNLSR TPETSCATQI KDFLLVIPFD CWEPVQE.
Here's a rearranged version of the original text:
APARSPSPST QPWEHVNAIQ EARRLLNLSR DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI ITFESFKENL KDFLLVIPFD CWEPVQE.
And here's another version with the words shuffled:
IPTFSSFENL EPTCLQTRLE LYKQGLRGSL ASHYKQHCPP DTAAEMNETV APARSPSPST EVISEMFDLQ QPWEHVNAIQ TKLKGPLTMM EARRLLNLSR TPETSCATQI KDFLLVIPFD CWEPVQE.

Concentration

> 98 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that Recombinant Human GM-CSF contains less than 1.0 EU/μg. This means that the product is of superior quality and meets strict standards for biological purity. The low level of endotoxins ensures that it is safe to use in various applications. These results provide assurance that the product is reliable and consistent, making it an ideal choice for scientific research and pharmaceutical purposes.

Activity

Compared to the standard, it is fully biologically active. The level of similarity is high, indicating that it is nearly identical. The ED, which is a measure of effectiveness, is consistent with the original substance.₅₀The specific activity of less than 0.1 ng/ml, as determined by a cell proliferation assay using human TF-1 cells, indicates a highly potent effect. This indicates that the concentration necessary to elicit a response in the cells is extremely low. A specific activity of > 1.0 × 10 highlights the remarkable potency of the substance being tested. This indicates that even at very minute concentrations, the substance is able to induce a significant response in the cells. The results of this assay demonstrate the strong biological activity of the substance and emphasize its potential therapeutic value.⁷ IU/mg.

Reconstitution

We suggest that prior to opening the vial, a brief centrifugation is required to settle the contents at the bottom. To achieve a concentration of 0.1-1.0 mg/mL, reconstitute in sterile distilled water or aqueous buffer with 0.1% BSA. Stock solutions must be divided into working aliquots and stored below -20 °C. Further dilutions should be carried out in buffered solutions appropriate to the experiment.

Stability & Storage

For optimum storage conditions, this lyophilized preparation can be stored at a temperature range of 2-8 °C. However, for long term storage, it is recommended to keep it at -20 °C. It is preferable to store the preparation in a desiccated form. After reconstitution, the stability of the preparation is maintained for up to one week when stored at 2-8 °C. To ensure maximum stability, it is advised to divide the reconstituted preparation into smaller working aliquots and store them at temperatures ranging from -20 °C to -70 °C. It is essential to avoid subjecting the preparation to repeated freeze/thaw cycles.

Target

Accession

P04141

Gene IDs

1437

References

References

1. Wang JM, Chen ZG, Colotta F, et al. 1988. Behring Inst Mitt: 270-3.

2. 1989. N Engl J Med, 320: 253-4.

3. Nissen-Druey C. 1989. Nouv Rev Fr Hematol, 31: 99-101.

4. Eager RandNemunaitis J. 2005. Mol Ther, 12: 18-27.

5. Tran T, Fernandes DJ, Schuliga M, et al. 2005. Br J Pharmacol, 145: 123-31.