Recombinant Mouse MIP-1β/CCL4 #abs01170

abs01170-2ug

1-2 Weeks

138

abs01170-10ug

1-2 Weeks

344

abs01170-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

A concentrated solution in 2 × PBS, pH 7.4 was filtered through a 0.2 μm filter and then lyophilized to produce the final product.

Properties

aa_sequence

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Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method determined that the concentration of rMuMIP-1β/CCL4 is lower than 1 EU/μg. Let me generate a highly similar content by rearranging the information: "According to the LAL method, the level of rMuMIP-1β/CCL4 was found to be less than 1 EU/μg in the sample."

Activity

The standard and its fully biologically active counterpart were compared, and the biological activity was assessed using human monocytes in a chemotaxis bioassay. The concentration range for observing this biological activity is 20-100 ng/ml.

Reconstitution

It is recommended to briefly centrifuge this vial before opening to ensure that the contents settle to the bottom. Reconstitution should be done using sterile distilled water or an appropriate aqueous buffer that includes 0.1% BSA, bringing the concentration to a range of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and stored at a temperature of ≤ -20 °C. Additional dilutions may be made using an appropriate buffered solution.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it should be kept at -20 °C, ideally in a desiccated state. Upon reconstitution, the preparation can be stored at 2-8 °C for up to one week. To ensure maximum stability, it is advisable to divide the reconstituted preparation into working aliquots and freeze them at a temperature ranging from -20 °C to -70 °C. It's crucial to avoid subjecting it to repeated freeze/thaw cycles since that can compromise its stability and effectiveness.

Target

Accession

P14097

Gene IDs

20303

References

References

1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.

2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.

3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.