Recombinant Mouse RANTES/CCL5 #abs01171

abs01171-5ug

1-2 Weeks

138

abs01171-20ug

1-2 Weeks

344

abs01171-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution was filtered through a 0.2 μm filter and then lyophilized. The lyophilization process was carried out using a mixture of 30 % Acetonitrile and 0.1 % TFA.

Properties

aa_sequence

The given content is a random series of letters and does not provide any meaningful information. Therefore, it is not possible to generate a highly similar content based on the original text information.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

As determined by the LAL method, the content expresses that the concentration of rMuRANTES/CCL5 is below 1 EU/μg. In order to generate highly similar content while rearranging the information, we can say:
"The LAL method determines that the rMuRANTES/CCL5 concentration is less than 1 EU/μg."

Activity

The standard and the fully biologically active form exhibit comparable levels of biological activity. In order to assess the biological activity, a chemotaxis bioassay using human T lymphocytes was conducted. The concentration range for this assay was determined to be 1.0-10 ng/ml.

Reconstitution

To ensure proper handling of this vial, we suggest that it be briefly spun down before opening to settle its contents to the bottom. For reconstitution, use sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/mL. Stock solutions should be divided into working aliquots and kept at or below -20°C. For further dilutions, employ suitable buffered solutions.

Stability & Storage

To maintain the stability of the lyophilized preparation, it is recommended to store it at 2-8°C. However, for long-term storage, it should be kept at -20°C, and it's best to keep it in a desiccated form. Once reconstituted, the preparation can remain stable for up to one week if kept at 2-8°C. To ensure maximum stability, it's advisable to divide the reconstituted preparation into smaller working aliquots and store them at -20°C to -70°C. Avoiding repeated freeze/thaw cycles is crucial in maintaining the quality of the preparation.

Target

Accession

P30882

Gene IDs

20304

References

References

1. Donlon TA, Krensky AM, Wallace MR, et al. 1990. Genomics. 6:548-53.

2. Maghazachi AA, Al-Aoukaty A, Schall TJ. 1996. Eur J Immunol. 26:315-9.

3. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5.