Recombinant Mouse C10/CCL6 #abs01172

abs01172-2ug

1-2 Weeks

138

abs01172-10ug

1-2 Weeks

344

abs01172-1mg

1-2 Weeks

3891

Species

Murine

Source

Escherichia coli.

Format

The concentrated solution in 20 mM Tris, pH 8.0 with 500 mM NaCl was filtered through a 0.2 μm filter and then lyophilized. A highly similar content can be generated by rephrasing this information such as: After filtration through a 0.2 μm filter, the solution with 20 mM Tris at pH 8.0 and 500 mM NaCl was concentrated and subjected to lyophilization for preservation.

Properties

aa_sequence

KVGIRSGPNQ HLQECITFQP YFPITFSCIY EDKCIEEILQ QSCQIDDPTF VIHRFPPSGY AFGKCIQEPG DAQVRRPCDS CRTQCSPKLG NRSVTSQGIY ISGPRRDKQF.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

The LAL method has determined that the rMuC10/CCL6 is present at levels less than 1 EU/μg. This information can be rearranged to create similar content that maintains the original meaning.

Activity

The standard and the fully biologically active substance were compared, and it was found that the biologically activity of the substance was equivalent to the standard. The determination of its biological activity was based on a chemotaxis bioassay using human CCR1 transfected murine BaF3 cells. The substance exhibited its maximum biological activity within a concentration range of 10-100 ng/ml.

Reconstitution

For optimal results, we suggest briefly centrifuging the vial prior to opening to ensure the contents settle to the bottom. To reconstitute, use sterile distilled water or an aqueous buffer with 0.1% BSA to achieve a concentration of 0.1-1.0 mg/ml. It is advisable to distribute stock solutions into working aliquots and store them at -20 °C or below. Dilutions should be carried out in appropriately buffered solutions.

Stability & Storage

To ensure the stability of the lyophilized preparation, it is recommended to keep it at 2-8 °C. However, for long term storage, it is best to store it at -20 °C, preferably desiccated. Once reconstituted, the preparation should be kept at 2-8 °C and is stable for up to one week. To prevent any degradation of the preparation, it is advisable to divide it into working aliquots and store them at -20 °C to -70 °C. Avoid exposing the preparation to repeated freeze/thaw cycles. These precautions will ensure that the preparation retains its efficacy and potency for optimal use.

Target

Accession

P27784

Gene IDs

20305

References

References

1. Berger MS, Kozak CA, Gabriel A, et al. 1993. DNA Cell Biol. 12:839-47.

2. Orlofsky A, Berger MS, Prystowsky MB. 1991. Cell Regul. 2:403-12.

3. Ma B, Zhu Z, Homer RJ, et al. 2004. J Immunol. 172:1872-81.