Recombinant Mouse Lymphotactin/XCL1 #abs01167

abs01167-5ug

1-2 Weeks

138

abs01167-20ug

1-2 Weeks

344

abs01167-1mg

1-2 Weeks

3891

Source

Escherichia coli.

Format

The concentrated solution in PBS, pH 7.4 was first filtered using a 0.2 μm filter before undergoing lyophilization. The resulting product was in a powder form.

Properties

aa_sequence

EVGVETESSL NTVCQTLRQL KPQIKTYIIW VGAMRAVIFV KERCATKGLI WPAAVVKEKAIT VDGTVRAKSTR ENMAKTPTVG AQRTSSTATI TGLLTEEPHSI.

Concentration

> 97 % by SDS-PAGE and HPLC analyses.

Endotoxin_level

According to the LAL method, the concentration of rMuLymphotactin/XCL1 is less than 0.1 EU/μg. It is important to note that this information is derived from the original text.

Activity

The potency of the substance is equivalent to that of the standard, indicating that it is fully biologically active. This result demonstrates that the substance is capable of producing the same effects as the standard, making it a viable alternative. The ED value further validates this finding and confirms that the substance is highly similar to the standard in terms of activity. Overall, these results are significant as they provide evidence for the potential use of the substance as a therapeutic agent.₅₀The specific activity of less than 100 ng/ml, as determined by a chemotaxis bioassay using human XCR1 transfected murine BaF3 cells, indicates a high potency of > 1.0 × 10. This finding suggests that the tested substance has a strong capability to stimulate chemotaxis and attract XCR1-expressing cells. The bioassay results highlight the potential biological activity and effectiveness of the substance in promoting cellular movement.⁴ IU/mg.

Reconstitution

Before opening the vial, we strongly recommend briefly centrifuging it to ensure the contents settle at the bottom. To reconstitute, add sterile distilled water or an aqueous buffer containing 0.1% BSA until the concentration reaches 0.1-1.0 mg/mL. It is advisable to divide the stock solutions into smaller working aliquots and store them at -20 °C or colder. When making further dilutions, always use appropriate buffered solutions.

Stability & Storage

To maintain the stability of this lyophilized preparation, it is recommended to store it at 2-8 °C. However, for long-term storage, it should be kept at -20 °C in a desiccated environment. Once reconstituted, the preparation can be used for up to one week when stored at 2-8 °C. For optimum stability, divide the reconstituted preparation into smaller working aliquots and store them at -20 °C to -70 °C. Avoid subjecting the preparation to repeated freeze/thaw cycles. Ensure that you properly manage the storage conditions of the reconstituted preparation to maintain its stability and efficacy.

Target

Accession

P47993

Gene IDs

16963