Rat IFN-γ High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs553807-96T	4 weeks	$580.00

Please be aware that the price stated is solely for your reference. In order to obtain the accurate price, kindly reach out to our seller Vecent as he is the only one who can provide you with the necessary details. However, rest assured that we strive to provide competitive pricing for our customers to ensure they receive the best value for their money. Thank you for considering our products and services.

Testing Principle：

The double antibody sandwich ELISA method is utilized in this kit. The conjugate plate comes prepackaged with a specific anti-human CXCL13 Capture Antibody, which exhibits high affinity. The process involves sequentially adding the standard substance, the sample to be tested, and the biotin labeled detection antibody into the HRP-plate well. After thoroughly oscillating and mixing, the sample containing CXCL13 is subjected to a 2-hour incubation at room temperature. During this incubation, the CXCL13 in the sample binds to both the solid phase antibody and the detection antibody.
To ensure accuracy, free and unbound constituents are removed via extensive washing. Subsequently, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. Following another round of washing, TMB color substrate is added and left to incubate in darkness at room temperature. The color development process occurs, and the depth of color reaction directly relates to the concentration of CXCL13 present in the sample.
To terminate the reaction, a termination solution is added. The absorbance value at a detection wavelength of 450 nm (with a correction wavelength of 570-630 nm) is measured using a microplate analyzer. This measurement provides valuable information about CXCL13 concentration.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：Included in the package is a pre-coated 96-well plate for your convenience. It also contains Standards for accurate comparison. Our CXCL13 test antibody is provided as a single copy, accompanied by Standard Diluent and Test Buffer for optimal sample preparation. To enable color development, we have included the TMB Color Substrate. For washing purposes, the package includes Washing Solution. The Stop Solution ensures the reaction is ceased at the desired time. To enhance detection, SA-HRP is also provided. Lastly, we have included a Seal Plate Film and detailed Instructions to assist you throughout the process.

Sensitivity: 0.26 pg/mL

Detection range： 3.13-200 pg/mL

Recovery rate： 73-137%

Storage：2-8℃

The standard curve：

 

Background：

IFN-γ is a soluble dimer cytokine involved in both innate and adaptive immunity. It is secreted by NK and NKT cells and also by CD4Th1 and CD8 cytotoxic T cells, specifically during antigen-specific immunity. Viral, bacterial, and protozoan infections are among the diseases that IFN-γ plays a vital role in fighting against. An important function of IFN-γ is activating macrophages and inducing type II MHCII expression, which enhances the immune response. Too much or too little IFN-γ expression is associated with autoimmune and autoinflammatory diseases. Aside from its direct inhibition of viral replication, IFN-γ also has immune-stimulating and immune-regulatory roles within the immune system.
IFN-γ is a marker of disease in many different pathological conditions, including infections, autoimmune diseases, transplant rejection, allergic reactions, and diabetes mellitus.

Hot Tags: rat ifn-γ high sensitivity elisa kit, China rat ifn-γ high sensitivity elisa kit suppliers