Mouse GM-CSF High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs552810-96T	4 weeks	$580.00

Important notice: The provided price is for reference only; please get in touch with our seller, Vecent, for detailed pricing information.

Testing Principle：

The double antibody sandwich ELISA method is utilized in this kit. The conjugate plate already contains the specific anti-human CXCL13 Capture Antibody with high affinity. Firstly, the standard substance, the sample to be tested, and the biotin labeled detection antibody are sequentially added into the HRP-plate well. The mixture is thoroughly oscillated and mixed to ensure proper interaction between the CXCL13 in the sample and the solid phase antibody as well as the detection antibody. Subsequently, the sample is incubated at room temperature for a duration of 2 hours. Following the incubation period, the mixture is thoroughly washed to eliminate any unbound ingredients. To detect the presence of the CXCL13, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced into the mixture. Another round of washing is performed before adding the TMB color substrate. The mixture is then incubated in a dark environment at room temperature, allowing the color to develop. The intensity of the color reaction is directly proportional to the concentration of CXCL13 present in the sample. To stop the reaction, a termination solution is added. Finally, the absorbance value is measured at a detection wavelength of 450 nm (with a correction wavelength of 570-630 nm) using a microplate analyzer.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：The package includes a pre-coated 96-well plate, a standards set, a single copy of CXCL13 test antibody, standard diluent, test buffer, TMB color substrate, washing solution, stop solution, SA-HRP, seal plate film, and a set of instructions. These components are carefully arranged to provide the necessary materials for conducting a CXCL13 test.

Sensitivity：0.23 pg/mL

Detection range： 2.5-160 pg/mL

Recovery rate： 91-112%

Storage：2-8℃

The standard curve：

 

Background：

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that plays a crucial role in regulating the growth and development of white blood cells. Its primary function is to stimulate the production of various types of white blood cells, including neutrophils, eosinophils, basophils, and monocytes, from stem cells. Moreover, GM-CSF exhibits activity against both hematopoietic and non-hematopoietic cells.
In the medical field, GM-CSF is utilized as a drug to stimulate the production of white blood cells, particularly in patients undergoing chemotherapy to prevent neutropenia, a condition characterized by abnormally low levels of neutrophils. This application helps safeguard patients against infections and maintain a healthy immune system during cancer treatment.
Excitingly, ongoing clinical trials are currently investigating the potential of GM-CSF as a vaccine adjuvant in individuals infected with HIV. Researchers aim to determine its effectiveness in enhancing the immune response and developing novel strategies for combatting this viral infection.
Notably, studies have observed elevated levels of GM-CSF in the blood serum of patients diagnosed with certain types of cancer, such as lung cancer and acute myeloid leukemia. This finding suggests a possible association between GM-CSF and the development or progression of these malignancies. Additionally, GM-CSF has been detected in the peripheral blood and scale extract of individuals suffering from bronchial asthma, implying its involvement in the pathogenesis or exacerbation of this respiratory condition.
In summary, GM-CSF serves as a vital cytokine in regulating the growth and activity of white blood cells, while also exhibiting effects on non-hematopoietic cells. Its clinical applications include stimulating white blood cell production during chemotherapy and potential usage as a vaccine adjuvant in HIV-infected individuals. The presence of high GM-CSF levels in specific cancers and bronchial asthma warrants further investigation into its role in these diseases.

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