Human IL-1β High Sensitivity ELISA Kit

SKU-Pack Size	Availability	Price
abs551801-96T	4 weeks	$580.00

Please note that the price provided is simply for your reference, to obtain detailed pricing information, please get in touch with our seller, Vecent. It is important to understand that the aforementioned price is subject to change and may not reflect the current pricing arrangement. We highly recommend contacting Vecent for an accurate and up-to-date pricing quote that meets your specific needs. Thank you.

Testing Principle：

The double antibody sandwich ELISA method is employed in this kit. The conjugate plate is pre-coated with a specific anti-human CXCL13 Capture Antibody of high affinity. The procedure starts by adding the standard substance, the sample to be tested, and the biotin-labeled detection antibody into the HRP-plate well successively. Following thorough oscillation and mixing, the sample is incubated at room temperature for 2 hours to allow CXCL13 to bind to the solid phase antibody and the detection antibody. After thorough washing to remove any unbound components, horseradish peroxidase labeled streptavidin-HRP (SA-HRP) is introduced. Another washing step is performed, and then a TMB color substrate is added and incubated in a dark environment at room temperature to initiate color development. The intensity of the color reaction is directly proportional to the concentration of CXCL13 present in the sample. Finally, a termination solution is added to stop the reaction, and the absorbance value is measured at a detection wavelength of 450 nm (with a correction wavelength of 570-630 nm) using a microplate analyzer.

Detection type：Double sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity：100 μl

Kit composition：Included in this package is a pre-coated 96-well plate for your convenience. We also provide standards for accurate comparison. You will find one copy of the CXCL13 test antibody for your experiment. The standard diluent and test buffer are included to optimize your assay conditions. Additionally, we offer the TMB color substrate for visualization purposes and a washing solution to ensure cleanliness. To stop the reaction, you will find the stop solution provided. The SA-HRP is also included in this package for detection purposes. Lastly, we provide a seal plate film to maintain the integrity of your samples. Detailed instructions are included to guide you through the process.

Sensitivity：0.02 pg/mL

Detection range： 0.19-12 pg/mL

Recovery rate： 93-112%

Storage：2-8℃

The standard curve：

 

Background：

Interleukin 1 (IL-1) is an extracellular peptide weighing 17kDa. It comprises two proteins, IL-1α and IL-1β. IL-1β is a protein precursor that is secreted by activated macrophages. It is converted into its activated form through hydrolysis of caspase1 (CASP1/ICE). The cytokine IL-1β plays a crucial role as a mediator of the inflammatory response, impacting diverse cellular activities such as cell proliferation, differentiation, and apoptosis. These IL-1 proteins are part of an important family derived from biologically activated monocytes. They play significant roles in inflammatory and immune responses. When IL-1β expression is elevated, it can lead to various autoinflammatory syndromes, particularly CAPS, which is a single-gene condition caused by mutations in the inflammasome receptor NLRP3, triggering IL-1β processing. To detect both the mature and unlysed precursor forms of human IL-1β, this kit is utilized effectively.

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