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Rabbit Anti-ZP1 Polyclonal Antibody#abs134943

Description

Catalog-specification	Delivery time	USD price
abs134943-100ug	1-2 Weeks	301.0
abs134943-50ug	1-2 Weeks	201.0

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Overview
catalog	abs134943
Other names	Zona pellucida glycoprotein 1 (ZP1) is a processed sperm-binding protein that serves as a critical receptor for the binding of sperm to the zona pellucida, a protective layer surrounding the oocyte. Also referred to as zona pellucida protein 1, ZP1 is essential for successful fertilization in mammals. The human ZP1 protein is encoded by the MGC87693 gene and is characterized by its high degree of similarity to other mammalian ZP1 proteins. As a fundamental component of the zona pellucida, ZP1 plays a vital role in facilitating sperm-egg interactions and ultimately enabling the formation of a viable embryo.
Source	Rabbit
Specificity	The total ZP1 levels can be effectively detected by the ZP1 Antibody, which targets endogenous ZP1 expression. Consequently, the antibody proves to be an excellent tool for analyzing the endogenous ZP1 protein levels in various biological samples.
Species Reactivity	Human;Mouse
Antigen	ZP1
Application	The dilutions required for Western blotting are typically in the range of 1:500 to 1:1000. For immunofluorescence or immunocytochemistry, the appropriate dilutions range from 1:100 to 1:500. When performing ELISA using a peptide antigen, the recommended dilutions are between 1:20000 and 1:40000. It is crucial to ensure that the generated content retains the essence of the original text, but it should be presented in a distinct manner without following the dialogue style of ChapGPT.
Immunogen	A synthesized peptide.
MW	70 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	It is advised to store at a temperature of -20 °C for a year while avoiding frequent freeze and thaw cycles.
Storage buffer	Our Rabbit IgG is formulated in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It is recommended to store the product at -20 °C, and it is stable for up to 12 months from the date of receipt. Order now and experience the benefits of our high-quality Rabbit IgG!
Target
Background	The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP1 ensures the structural integrity of the zona pellucida.
Tissue specificity	Oocytes.
Posttranslational modification	Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.O-glycosylated.
Celluar localization	Extracellular region or secreted;Golgi apparatus;Plasma Membrane;
UniPort	P60852