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Rabbit Anti-WTAP Polyclonal Antibody#abs133584

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Description

Catalog-specification	Delivery time	USD price
abs133584-100ug	1-2 Weeks	301.0
abs133584-50ug	1-2 Weeks	201.0

Overview
catalog	abs133584
Other names	The protein DKFZp686F20131, also known by various other names such as Female-lethal(2)D homolog, FL2D_HUMAN, hFL(2)D, KIAA0105, MGC3925, Mum2, OTTHUMP00000017522, OTTHUMP00000017523, PNAS 132, PNAS-132, PNAS132, is a putative pre-mRNA splicing regulator. It is a homolog of the female lethal 2D protein. It is also referred to as the Wilms tumor 1 associating protein or the WT1-associated protein. The protein is believed to play a role in pre-mRNA splicing regulation. Its exact function and mechanisms of action are still being studied. The protein is encoded by the WTAP gene. To generate highly similar content based on the original text, here's a rearranged version: The FL2D_HUMAN protein, also known as DKFZp686F20131, has various aliases such as hFL(2)D, KIAA0105, MGC3925, Mum2, OTTHUMP00000017522, OTTHUMP00000017523, PNAS 132, PNAS-132, PNAS132. It is a putative pre-mRNA splicing regulator and a homolog of the female lethal(2)D protein. Additionally, it is referred to as the Wilms tumor 1 associating protein or WT1-associated protein. The protein, encoded by the WTAP gene, is involved in the regulation of pre-mRNA splicing. Its precise function and mechanisms are still under investigation.
Source	Rabbit
Specificity	Total WTAP levels can be detected by the WTAP Antibody, which is designed to recognize the endogenous levels of WTAP protein. By using this antibody, researchers can accurately measure the total WTAP protein present in a biological sample.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Sheep
Antigen	WTAP
Application	For Western blotting, it is recommended to use a dilution of 1:500 to 1:1000. For immunofluorescence and immunocytochemistry, a dilution of 1:100 to 1:500 is appropriate. When performing ELISA using a peptide substrate, a dilution range of 1:20000 to 1:40000 is recommended. To recap, for best results in these assays, use the appropriate dilution as outlined above.
Immunogen	A synthesized peptide.
MW	44 KD
Properties
Concentration	1mg/ml
purification	Peptide affinity chromatography was employed to purify the antiserum using SulfoLink™ Coupling Resin. The resin was used as a tool to isolate the target peptides and remove any impurities from the antiserum. This method resulted in a highly purified antiserum suitable for its intended use.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Regulatory subunit of the WMM N6-methyltransferase complex, a multiprotein complex that mediates N6-methyladenosine (m6A) methylation of some adenosine residues of some mRNAs and plays a role in the efficiency of mRNA splicing, processing and mRNA stability. Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
Tissue specificity	Ubiquitously expressed.
Celluar localization	Nucleus;
UniPort	Q15007