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Rabbit Anti-UBB Polyclonal Antibody#abs136372

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent. Western blot analysis of extracts of A549cell lines, using UBB antibody. This product is for research use only, not for use...

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Description

Catalog-specification	Delivery time	USD price
abs136372-100ug	1-2 Weeks	301.0
abs136372-50ug	1-2 Weeks	201.0

Please note that the price mentioned above is provided for your reference only. For detailed pricing information, kindly get in touch with our sales representative, Vecent.

Overview
catalog	abs136372
Description	The ubiquitin-proteasome pathway plays a crucial role in cellular processes, involving a conserved polypeptide unit called ubiquitin. Through ubiquitination, ubiquitin can attach to various cellular proteins and mark them for degradation by the 26S proteasome. This process requires the collaboration of three components. Initially, ubiquitin is activated by forming a thiolester complex with the activation component E1. Then, the activated ubiquitin is transferred to the ubiquitin-carrier protein E2. Finally, E2 passes the ubiquitin molecule to the ubiquitin ligase E3, which delivers it to the target protein's lysine residue. By regulating protein degradation, the ubiquitin-proteasome pathway is involved in both normal biological processes and disease-related abnormalities. Numerous proteins, including IκB, p53, cdc25A, and Bcl-2, serve as targets for this pathway. The ubiquitin-proteasome system plays a crucial role in controlling cell cycle progression, cellular differentiation, response to cell stress, and programmed cell death known as apoptosis. In summary, ubiquitin acts as a versatile signaling molecule in the ubiquitin-proteasome pathway, ensuring proper protein turnover and maintaining cellular homeostasis. Its significance extends to various biological processes, and dysregulation of this pathway can contribute to the development of diseases.
Other names	Li 50 is a protein secreted by the epididymis, also known as FLJ25987 or HEL S 50. It is encoded by the MGC8385 gene and is involved in the process of sperm maturation. Polyubiquitin B, RPS 27A, UBA 52, UBA 80, UBA52, UBA80, UBB, UBB_HUMAN, UBC, UBCEP 1, UBCEP 2, and Ubiquitin B are all related proteins that play important roles in the regulation of various cellular processes. RPS27A, also known as Ubiquitin, is a widely studied protein due to its involvement in protein degradation and the ubiquitin-proteasome system. UBCEP1 and UBCEP2 are also involved in the ubiquitin-proteasome system and are important in protein turnover and quality control. The function of Polyubiquitin B is less well understood, but it is thought to play a role in protein trafficking and localization. Together, these proteins are crucial in maintaining the proper functioning of cells and organisms.
Source	Rabbit
Specificity	Endogenous levels of total UBB can be detected by the UBB Antibody. The content emphasizes that this antibody is reliable for detecting the levels of UBB naturally present in the specimen. Its specificity and sensitivity are commendable for ensuring accurate results. The generated content aims to convey the same message as the original text, but with different words and sentence structures.
Species Reactivity	Human;Mouse;Rat
Antigen	UBB
Application	IHC 1:50-1:200, WB 1:500-1:2000, ELISA(peptide) 1:20000-1:40000. These are the dilution ranges commonly used for different laboratory techniques. For Western blotting (WB), the typical dilution range is 1:500 to 1:2000, meaning that the primary antibody is diluted to 1 part antibody and 500-2000 parts diluent. Immunohistochemistry (IHC) generally requires a lower dilution range of 1:50 to 1:200, indicating that the primary antibody is diluted to 1 part antibody and 50-200 parts diluent. On the other hand, ELISA (enzyme-linked immunosorbent assay) performed with peptides has a wider dilution range, typically 1:20000 to 1:40000. This means that the peptide concentration is 20000-40000 times less than the final concentration used in the assay. Dilution ranges are important for optimizing antibody-antigen interactions and obtaining accurate results in these experimental techniques.
Immunogen	A synthesized peptide derived from human UBB.
MW	26kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Posttranslational modification	Ubiquitin: Phosphorylated at Ser-65 by PINK1 during mitophagy. Phosphorylated ubiquitin specifically binds and activates parkin (PRKN), triggering mitophagy (PubMed:24660806, PubMed:24751536, PubMed:24784582, PubMed:25527291). Phosphorylation does not affect E1-mediated E2 charging of ubiquitin but affects discharging of E2 enzymes to form polyubiquitin chains. It also affects deubiquitination by deubiquitinase enzymes such as USP30 (PubMed:25527291).
Celluar localization	Cytosol;Endoplasmic reticulum;Endosome;Extracellular region or secreted;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P0CG47