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Rabbit Anti-TNFAIP3 Polyclonal Antibody#abs136370

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Description

Catalog-specification	Delivery time	USD price
abs136370-100ug	1-2 Weeks	301.0
abs136370-50ug	1-2 Weeks	201.0

Overview
catalog	abs136370
Description	A20 is a protein that is induced by cytokines and plays a crucial role in inhibiting apoptosis while promoting NF-κB activation. It was first discovered in human umbilical vein endothelial cells as a TNF-α-inducible primary response gene, and its sequence shows that it contains seven Cys2/Cys2-zinc finger motifs. Constitutive expression of A20 is observed in lymphoid tissues, but stressful stimuli such as TNF-α, IL-1, phorbol esters, and LPS transiently induce its expression. By interfering with TRAF family members and interacting with the NF-κB inhibiting protein ABIN, A20 is able to confer resistance to apoptosis and NF-κB activation. Additionally, A20 has site-specific ubiquitin modifying activity that contributes to its biological functions. Its amino-terminus contains DUB activity for Lys63 branches, while the carboxyl-terminus contains E3 activity for Lys48 branches, leading to the degradation of TRAF6 and RIP substrates. Overall, A20 plays a critical role in regulating the immune response and inflammation.
Other names	Protein A20, also known as TNFAIP3, is a putative DNA-binding protein that contains the OTU domain. It is encoded by the MGC104522, MGC138687, and MGC138688 genes. A20 is involved in various biological processes and is regulated by TNF alpha. Its function as a tumor necrosis factor-induced protein 3 makes it an important player in immune responses. With its zinc finger domain, A20 exhibits DNA binding abilities.
Source	Rabbit
Specificity	The TNFAIP3 Antibody is designed to target total TNFAIP3 levels present within the body. It detects the endogenous levels of the protein, allowing for accurate measurement and analysis. It is a valuable tool for research, providing a reliable means of studying TNFAIP3 and its role in various biological processes. By utilizing this antibody, scientists can gain a better understanding of the function and significance of TNFAIP3 in the body.
Species Reactivity	Human;Mouse;Rat
Antigen	TNFAIP3
Application	WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human TNFAIP3.
MW	90kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Ubiquitin-editing enzyme that contains both ubiquitin ligase and deubiquitinase activities. Involved in immune and inflammatory responses signaled by cytokines, such as TNF-alpha and IL-1 beta, or pathogens via Toll-like receptors (TLRs) through terminating NF-kappa-B activity. Essential component of a ubiquitin-editing protein complex, comprising also RNF11, ITCH and TAX1BP1, that ensures the transient nature of inflammatory signaling pathways. In cooperation with TAX1BP1 promotes disassembly of E2-E3 ubiquitin protein ligase complexes in IL-1R and TNFR-1 pathways; affected are at least E3 ligases TRAF6, TRAF2 and BIRC2, and E2 ubiquitin-conjugating enzymes UBE2N and UBE2D3. In cooperation with TAX1BP1 promotes ubiquitination of UBE2N and proteasomal degradation of UBE2N and UBE2D3. Upon TNF stimulation, deubiquitinates 'Lys-63'-polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteasomal degradation and consequently termination of the TNF- or LPS-mediated activation of NF-kappa-B. Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation. Targets TRAF2 for lysosomal degradation. In vitro able to deubiquitinate 'Lys-11'-, 'Lys-48'- and 'Lys-63' polyubiquitin chains. Inhibitor of programmed cell death. Has a role in the function of the lymphoid system. Required for LPS-induced production of proinflammatory cytokines and IFN beta in LPS-tolerized macrophages.
Tissue specificity	By TNF.
Posttranslational modification	Proteolytically cleaved by MALT1 upon TCR stimulation; disrupts NF-kappa-B inhibitory function and results in increased IL-2 production. It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact.
Celluar localization	Cytosol;Extracellular region or secreted;Lysosome;Nucleus;
UniPort	P21580