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Rabbit Anti-SPTBN1 Polyclonal Antibody#abs137818

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Description

Catalog-specification	Delivery time	USD price
abs137818-100ug	1-2 Weeks	301.0
abs137818-50ug	1-2 Weeks	201.0

Overview
catalog	abs137818
Other names	Some possible variations of the content are: - Beta spectrin, also known as spectrin beta chain or SPTBN1, is a protein involved in cell membrane structure and function. It exists in several isoforms, including Beta G spectrin, Beta II spectrin, and Beta spectrin 2 (or Beta-II spectrin). - ELF (embryonic liver beta fodrin) is another name for Beta spectrin 2, which is mainly expressed in developing tissues and in certain cancer cells. - Fodrin beta chain is the name given to the beta subunit of the spectrin molecule that forms a heterodimer with alpha subunit to create the functional spectrin tetramer. The non-erythroid beta chain 1 variant refers to the beta subunit found in non-red blood cells. - Spectrin is a structural protein that forms a meshwork underneath the plasma membrane of many eukaryotic cells, providing stability, elasticity, and resistance to mechanical stress. - The genetic code of human SPTBN1 gene encodes a polypeptide of 2,182 amino acids that undergoes several post-translational modifications, including phosphorylation and cleavage, to generate the mature form of Beta spectrin. - Mutations in SPTBN1 have been associated with several human diseases, including hereditary spherocytosis (a type of hemolytic anemia), spinocerebellar ataxia type 5 (a neurodegenerative disorder), and some forms of cancer. Understanding the molecular basis of these disorders can help develop effective therapies targeting Beta spectrin.
Source	Rabbit
Specificity	The total levels of endogenous SPTBN1 can be effectively detected using the SPTBN1 Antibody.
Species Reactivity	Human
Antigen	SPTBN1
Application	ELISA (peptide): The recommended dilution range for ELISA using peptide as the antigen is 1:20,000 to 1:40,000. In this assay, the peptide is immobilized on a solid surface, allowing specific antibodies to bind to it and be detected. WB (Western Blot): The recommended dilution range for WB using a protein sample is 1:1,000 to 1:3,000. This technique involves separating proteins based on their molecular weight and then transferring them onto a membrane for antibody detection. IF/ICC (Immunofluorescence/Immunocytochemistry): The recommended dilution range for IF/ICC is 1:100 to 1:500. This method involves the use of fluorescently labeled antibodies to visualize specific proteins or antigens within cells or tissues. It is important to note that the dilution ranges provided are general guidelines and may need to be optimized for specific experimental conditions.
Immunogen	A synthesized peptide derived from human SPTBN1.
MW	275 kDa
Properties
Concentration	1mg/ml
purification	We utilized SulfoLink™ Coupling Resin in peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	For optimal storage, keep at a temperature of -20 °C and avoid exposing the product to frequent freeze/thaw cycles. This storage condition is recommended for a duration of one year. It is important to note that any deviation from these instructions may negatively impact the quality and efficacy of the product.
Storage buffer	Phosphate-buffered saline, pH 7.4, containing rabbit IgG, 150mM NaCl, 0.02% sodium azide, and 50% glycerol should be stored at -20°C. This solution remains stable for a period of 12 months starting from the date of receipt.
Target
Background	Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
Tissue specificity	Isoform 2 is present in brain, lung and kidney (at protein level).
Celluar localization	Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;
UniPort	Q01082