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Rabbit Anti-SIAH1 Polyclonal Antibody#abs133643

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Description

Catalog-specification	Delivery time	USD price
abs133643-50ug	1-2 Weeks	201.0
abs133643-100ug	1-2 Weeks	301.0

Overview
catalog	abs133643
Other names	The E3 ubiquitin-protein ligases known as SIAH1 and SIAH2 are important enzymes involved in a variety of cellular processes, including cell proliferation and apoptosis. SIAH1 and SIAH2 are also known as Seven in absentia homolog 1 and 2, respectively, in reference to their role as homologs of the Drosophila protein Seven in absentia. These proteins play a key role in the targeted degradation of specific proteins via the ubiquitin-proteasome system. There are several aliases for SIAH1 and SIAH2 including FLJ08065, hSIAH1, HUMSIAH, Siah 1, Siah 1a, Siah1a, and SIAH1_HUMAN, among others. The various names reflect their importance in different cellular pathways and their potential as therapeutic targets for various diseases including cancer. Despite their similarity, SIAH1 and SIAH2 also have distinct functions that are specific to their individual roles in cellular signaling and protein degradation. Understanding the mechanisms and functions of these enzymes is an important area of research that has implications for the development of novel treatments for a range of diseases.
Source	Rabbit
Specificity	The detection of endogenous levels of total SIAH1 is accomplished by using the SIAH1 Antibody. This antibody specifically targets and recognizes the entire pool of SIAH1 protein present in the cells. By utilizing this antibody, researchers are able to quantitatively measure the amount of SIAH1 in a given sample. This information is crucial for understanding the regulation and function of SIAH1 in biological processes.
Species Reactivity	Human;Mouse;Rat
Antigen	SIAH1
Application	The dilution range for Western blot is 1:500 to 1:1000, for immunofluorescence/immunocytochemistry is 1:100 to 1:500, and for ELISA using peptides is 1:20000 to 1:40000.
Immunogen	A synthesized peptide.
MW	34 KD
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	To ensure optimal storage, it is recommended to keep the product at a temperature of -20 °C for up to one year. It is essential to avoid subjecting the product to repeated freeze/thaw cycles, as this can cause potential damage to the product. So, it is advisable to store it properly to maintain its quality and effectiveness.
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
Tissue specificity	Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
Posttranslational modification	Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
Celluar localization	Cytosol;Endosome;Nucleus;Plasma Membrane;
UniPort	Q8IUQ4/O43255