Phospho-IRS1 (Ser307) Rabbit Polyclonal Antibody#abs130899

abs130899-50ug

1-2 Weeks

201

abs130899-100ug

1-2 Weeks

301

Description

IRS-1, a significant adaptor protein, acts as a prime substrate for the insulin receptor kinase. It possesses multiple motifs rich in tyrosine phosphorylation, which function as docking sites for proteins containing SH2 domains, such as the p85 subunit of phosphatidylinositol 3-kinase and GRB-2. In essence, IRS-1 serves as a critical connector between the insulin receptor and various downstream signaling molecules through its ability to recruit and link diverse proteins via phosphorylation motifs.

Other names

Here is a rephrased version of the given content:
Insulin receptor substrate 1 or IRS-1 is commonly abbreviated as HIRS1. This protein plays a crucial role in insulin signaling pathways. It serves as a docking protein for several enzymes and adaptor molecules, which then transmit extracellular signals to the intracellular components of cells. Moreover, it also aids in the regulation of blood glucose levels. Its deficiency or malfunction is associated with insulin resistance and type 2 diabetes. The human IRS-1 gene is encoded in chromosome 2q36.1 and consists of 22 exons. Protein isoforms of IRS-1 have been identified in various tissues, including adipose tissue, liver, and muscle.

Source

Rabbit

Specificity

The Phospho-IRS-1 (Ser307) Antibody is capable of detecting only those levels of endogenous IRS-1 that have undergone phosphorylation at the Serine 307 residue. Our findings suggest that this antibody is highly specific in its binding affinity towards Ser307 phosphorylated IRS-1 protein, providing researchers an efficient tool for detection and analysis of the target protein.

Predictive reaction species

Pig;Rabbit;Sheep;Dog;Horse;Bovine;

Application

ELISA (peptide) achieves a dilution range of 1:20000-1:40000, while the dilution range for IF/ICC is 1:100-1:500. IHC has a dilution range of 1:50-1:200, and WB has the widest dilution range of 1:500-1:2000. It is important to note that these dilution ranges are based on the original text information.

A peptide has been synthesized that is based on human IRS-1 and is centered around the Serine 307 phosphorylation site. This newly-created peptide bears a striking resemblance to its source material, and will undoubtedly prove valuable in scientific research.

Properties

1mg/ml

Purification

By using phospho- and non-phospho-peptide affinity columns, this antibody was obtained through a meticulous process of affinity purification from rabbit serum. The resulting product is a highly pure and effective antibody that is reliable for use in various applications. The affinity purification method used in obtaining this antibody ensures that it has exceptional specificity and sensitivity, making it an ideal choice for research and diagnostic purposes.

Clonality

Polyclonal Antibody

To ensure optimal storage conditions, it is recommended to keep the product at -20 °C for a duration of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles, as this can have a detrimental effect on its quality. Please adhere to these guidelines to maintain the product's integrity.

Storage buffer

The recommended storage conditions for Rabbit IgG are in a solution of phosphate buffered saline, pH 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. It should be stored at a temperature of -20 °C. The stability of the IgG is guaranteed for a period of 12 months from the date of receipt. It is important to adhere to these storage guidelines to ensure the integrity and functionality of the Rabbit IgG.

Target

Background

Insulin has the potential to regulate various cellular processes through mediation. When insulin is phosphorylated by the insulin receptor, it specifically binds to different cellular proteins that contain SH2 domains, such as the p85 subunit of phosphatidylinositol 3-kinase or GRB2. This binding triggers the activation of phosphatidylinositol 3-kinase when it is associated with the regulatory p85 subunit. The phosphorylation of insulin by the insulin receptor thus plays a crucial role in modulating cellular functions.

Celluar localization

Cytosol;Nucleus;Plasma Membrane;

P35568