Rabbit Anti-TUBA3C/E Polyclonal Antibody#abs135029
Please note that the price provided above is for your reference only. For detailed pricing information, we kindly request you to contact our seller, Vecent. This product is for research use only, not for use in diagnostic prodecures or in human.
Description
| Catalog-specification | Delivery time | USD price |
abs135029-100ug | 1-2 Weeks | 301.0 |
abs135029-50ug | 1-2 Weeks | 201.0 |
Please note that the price provided above is for your reference only. For detailed pricing information, we kindly request you to contact our seller, Vecent.
| Overview | |
catalog | abs135029 |
| Other names | There are several aliases and isotypes for alpha-tubulin, including Alpha-tubulin 2, Alpha-tubulin 3C/D, alpha-tubulin isotype H2-alpha, H2-ALPHA, TBA3C_HUMAN, TUBA2, TUBA3C, TUBA3D, Tubulin alpha 2, Tubulin alpha 2 chain, Tubulin alpha 3c, Tubulin alpha-2 chain, and Tubulin alpha-3C/D chain. Additionally, there is Alpha-tubulin 3, H2 ALPHA, TBA1A_HUMAN, TUBA1A, TUBA3C, TUBA3D, TUBA3E, Tubulin alpha 3E chain, Tubulin alpha-1A chain, Tubulin alpha-3 chain, Tubulin B-alpha-1, Tubulin, alpha 1a, Tubulin, alpha 1b, Tubulin, alpha 1c, Tubulin, alpha 3c, and Tubulin, alpha 3e. Despite these different aliases, all of these proteins are essentially different names for similar proteins that make up part of the microtubules in cells. |
| Source | Rabbit |
| Specificity | The detection capability of TUBA3C/E Antibody extends to the endogenous levels of total TUBA3C/E. The antibody is specifically designed to target and identify the overall amount of TUBA3C/E present. We can rely on this antibody to accurately measure the levels of TUBA3C/E in biological samples. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | TUBA3C/E |
| Application | The recommended dilution range for WB is 1:500 to 1:1000, while for IF/ICC it is 1:100 to 1:500. For ELISA using peptide, the recommended dilution range is 1:20000 to 1:40000. It is important to note that these dilution ranges are based on the original text information and should be followed carefully for optimal results. |
| Immunogen | A synthesized peptide. |
| MW | 50 KD |
| Properties | |
Concentration | 1mg/ml |
| purification | To obtain a purified antiserum, SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography. This technique was employed to ensure efficient purification of the antiserum based on the original text information. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To maintain preservation, it is advised to store the product at a temperature of -20 °C for a duration of one year. It is crucial to prevent the product from undergoing repeated freeze/thaw cycles. |
| Storage buffer | This solution contains Rabbit IgG in phosphate buffered saline with a pH of 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol and should be stored at -20°C. It is stable for up to 12 months from the date of receipt. |
Target | |
Background | Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. |
| Tissue specificity | Testis specific. |
| Posttranslational modification | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).Acetylation of alpha chains at Lys-40 is located inside the microtubule lumen. This modification has been correlated with increased microtubule stability, intracellular transport and ciliary assembly.Methylation of alpha chains at Lys-40 is found in mitotic microtubules and is required for normal mitosis and cytokinesis contributing to genomic stability.Nitration of Tyr-450 is irreversible and interferes with normal dynein intracellular distribution.Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.Tubulin alpha-1B chain: Tyrosination promotes microtubule interaction with CAP-Gly domain-containing proteins such as CLIP1, CLIP2 and DCTN1 (By similarity). Tyrosination regulates the initiation of dynein-dynactin motility via interaction with DCTN1, which brings the dynein-dynactin complex into contact with microtubules (PubMed:26972003). In neurons, tyrosinated tubulins mediate the initiation of retrograde vesicle transport (By similarity).Detyrosinated tubulin alpha-1C chain: Detyrosination is involved in metaphase plate congression by guiding chromosomes during mitosis: detyrosination promotes interaction with CENPE, promoting pole-proximal transport of chromosomes toward the equator (PubMed:25908662). Detyrosination increases microtubules-dependent mechanotransduction in dystrophic cardiac and skeletal muscle. In cardiomyocytes, detyrosinated microtubules are required to resist to contractile compression during contraction: detyrosination promotes association with desmin (DES) at force-generating sarcomeres, leading to buckled microtubules and mechanical resistance to contraction (By similarity). |
| Celluar localization | Cytoskeleton;Nucleus; |
| UniPort | Q13748/Q6PEY2 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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