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Rabbit Anti-TLR1 Polyclonal Antibody#abs135699

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Description

Catalog-specification	Delivery time	USD price
abs135699-100ug	1-2 Weeks	301.0
abs135699-50ug	1-2 Weeks	201.0

Overview
catalog	abs135699
Description	TLRs, which are named after the closely related Toll receptor in Drosophila, play a critical role in innate immune responses. They are responsible for recognizing conserved motifs present in different pathogens and initiating defense responses. Activation of the TLR pathway results in the activation of NF-κB, which in turn regulates immune and inflammatory genes. The IL-1 receptor family members also share a conserved region called the TIR domain, comprising approximately 200 amino acids. TLRs bind to cytoplasmic adaptor proteins containing TIR domains, such as MyD88, MAL/TIRAP, TRIF, and TRAM. These associations lead to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK. IKK activation causes the degradation of IκB, a protein that normally keeps NF-κB inactive by sequestering it in the cytoplasm. Toll-like receptor expression is highest in peripheral blood leukocytes, monocytes, and macrophages, although TLR1 may have a broader expression pattern compared to other family members. TLR1 works in cooperation with TLR2 to recognize bacterial lipoproteins and activate NF-κB. Interestingly, TLR1 shares the highest sequence identity (69%) with TLR6.
Other names	Antigen CD281, also known as CD281 or KIAA0012, is a protein that is encoded by the TLR1 gene. It is also referred to as LPRS5, rsc786, TIL, or TIL. LPRS5. CD281 is a member of the Toll-like receptor (TLR) family, which plays a crucial role in immune responses. Toll-like receptors are a group of proteins that are involved in recognizing and responding to pathogen-associated molecular patterns (PAMPs). These receptors are evolutionarily conserved and are found in both insects (e.g., Toll protein in Drosophila) and mammals (e.g., TLR1 in humans). CD281 is specifically involved in recognizing and responding to lipopeptides, which are components of bacterial cell walls. Upon binding to lipopeptides, CD281 activates downstream signaling pathways that lead to the production of pro-inflammatory cytokines and the activation of immune cells. The activation of CD281 and other Toll-like receptors is crucial for the host defense against microbial infections. These receptors play a sentinel role by detecting the presence of pathogens and initiating an immune response to eliminate them. In addition to their role in innate immunity, Toll-like receptors have also been implicated in various other biological processes, including development, tissue repair, and the pathogenesis of autoimmune and inflammatory diseases. Overall, CD281, or Toll-like receptor 1, is an important protein involved in the recognition of lipopeptides and the initiation of immune responses. Its role in immune defense highlights its significance in maintaining overall health and combating infections.
Source	Rabbit
Specificity	Endogenous levels of total TLR1 can be accurately detected using the TLR1 Antibody. The antibody specifically targets and binds to the endogenous TLR1 protein, enabling the quantification of its total expression levels within the sample. This detection method is highly reliable and allows researchers to study the abundance and distribution of TLR1 in various biological systems. By utilizing the TLR1 Antibody, valuable insights can be gained into the functioning and regulation of TLR1, contributing to a better understanding of immune responses and the development of therapeutic strategies.
Species Reactivity	Human;Mouse;Rat
Antigen	TLR1
Application	The dilution ratios for Western blot (WB) can range from 1:500 to 1:2000, while for immunohistochemistry (IHC), the recommended dilution is between 1:50 and 1:200. For ELISA with peptide detection, the dilution range is typically 1:20000 to 1:40000. These dilution ratios are important factors to consider when performing these assays as they determine the optimal concentration of the primary antibody for accurate and reliable results.
Immunogen	A synthesized peptide derived from human TLR1.
MW	90kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Participates in the innate immune response to microbial agents. Specifically recognizes diacylated and triacylated lipopeptides. Cooperates with TLR2 to mediate the innate immune response to bacterial lipoproteins or lipopeptides (PubMed:21078852). Forms the activation cluster TLR2:TLR1:CD14 in response to triacylated lipopeptides, this cluster triggers signaling from the cell surface and subsequently is targeted to the Golgi in a lipid-raft dependent pathway (PubMed:16880211). Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.
Tissue specificity	Ubiquitous. Highly expressed in spleen, ovary, peripheral blood leukocytes, thymus and small intestine.
Celluar localization	Golgi apparatus;Plasma Membrane;
UniPort	Q15399