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Rabbit Anti-ST5 Polyclonal Antibody#abs133528

Description

Catalog-specification	Delivery time	USD price
abs133528-100ug	1-2 Weeks	301.0
abs133528-50ug	1-2 Weeks	201.0

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Overview
catalog	abs133528
Other names	Suppression of tumorigenicity 5 protein, also known as ST5, HeLa tumor suppression 1, or p126, is encoded by the DENND2B gene. DENND2B belongs to the DENN domain-containing protein family and contains a DENN/MADD domain that is involved in membrane trafficking and signaling. This protein has been shown to play a role in tumor suppression and is commonly expressed in various tissues and cell types. Its precise function is still under investigation, and further research into the mechanisms of DENND2B may reveal new insights into the development and progression of cancer.
Source	Rabbit
Specificity	The total ST5 levels in the body can be detected using the ST5 Antibody, which is capable of identifying endogenous levels of this protein. By rearranging the information provided, it can be stated that the ST5 Antibody is effective in detecting the total ST5 levels naturally present in the body.
Species Reactivity	Human;Mouse
Predictive reaction species	Horse;Rabbit;
Antigen	ST5
Application	，。，，： ELISA(peptide) 1:20000-1:40000 is a widely used technique in research. It is often used to detect and quantify specific peptides in samples. The dilution range for this assay is typically between 1:20000 and 1:40000. WB 1:500~1:1000, on the other hand, is a method used to analyze protein expression levels. This technique is based on the principle of gel electrophoresis and can provide information about protein size and abundance. The recommended dilution range for WB is between 1:500 and 1:1000. IF/ICC 1:100-1:500 is commonly used for fluorescence-based imaging of cells. This technique allows researchers to visualize specific proteins within cells. The suggested dilution range for IF/ICC is between 1:100 and 1:500. These dilution ranges provide optimal conditions for accurate and reliable results. It is important to carefully consider the specific assay and target before determining the appropriate dilutions for your experiments.
Immunogen	A synthesized peptide.
MW	126 KD
Properties
Concentration	1mg/ml
purification	We used SulfoLink™ Coupling Resin to perform peptide affinity chromatography and purify the antiserum. Let me rearrange the content to ensure it is based on the original information: The purification method employed for the antiserum involved peptide affinity chromatography using SulfoLink™ Coupling Resin.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality and effectiveness of the product, it is recommended to store it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated freeze/thaw cycles as this may compromise its stability and potency. By following these storage guidelines, you can ensure that the product remains optimal for use when needed.
Storage buffer	Phosphate-buffered saline containing Rabbit IgG is an aqueous solution with a pH of 7.4. It contains 150mM NaCl and 0.02% sodium azide as preservatives. Additionally, the solution includes 50% glycerol as a stabilizing agent. It is recommended to store this solution at a temperature of -20 °C. The solution remains stable for a period of 12 months from the date of receipt.
Target
Background	Guanine nucleotide exchange factor (GEF) which may activate RAB9A and RAB9B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. May be involved in cytoskeletal organization and tumorogenicity. Isoform 1 seems to be involved in a signaling transduction pathway leading to activation of MAPK1/ERK2. Isoform 3 may block ERK2 activation stimulated by ABL1. Isoform 3 may alter cell morphology and cell growth.
Tissue specificity	Widely expressed with the exception of peripheral blood lymphocytes. Isoform 1 is expressed in several epithelial and fibroblast (including tumorigenic) but absent in lymphoid cell lines (at protein level). Isoform 3 is expressed in primary cell or weakly tumorigenic but not in tumorigenic cell lines (at protein level).
UniPort	P78524