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Rabbit Anti-Sin3B Polyclonal Antibody#abs140553

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Description

Catalog-specification	Delivery time	USD price
abs140553-100ug	1-2 Weeks	301.0
abs140553-50ug	1-2 Weeks	201.0

Overview
catalog	abs140553
Other names	Sin3b is a subunit of the histone deacetylase complex. It is also known as the paired amphipathic helix protein Sin3b and belongs to the SIN3 transcription regulator family. Sin3b is involved in transcriptional regulation and acts as a corepressor to regulate gene expression. It is an important transcriptional regulator and homolog of SIN3 that plays a vital role in controlling gene expression. With its unique properties and structure, Sin3b has become the focus of intense study in the fields of genetics and molecular biology. Its functions and mechanisms of action are still being explored and understood, providing promising avenues for further research into this important cellular protein.
Source	Rabbit
Specificity	The Sin3B Antibody is capable of identifying total Sin3B levels present in the body. This detection is applicable for endogenous levels of Sin3B. To generate similar content based on the original text, one could state that the Sin3B Antibody recognizes the total Sin3B levels in the body, which are of endogenous origin.
Species Reactivity	Human;Mouse;Rat
Antigen	Sin3B
Application	According to the original information, the dilution ratios for WB, IHC, IF/ICC, and ELISA(peptide) are 1:1000, 1:200, 1:100-1:500, and 1:20000-1:40000 respectively. It is important to follow these dilution guidelines to obtain accurate results. For Western blotting, a dilution ratio of 1:1000 is recommended. For immunohistochemistry, a dilution ratio of 1:200 is suggested. For immunofluorescence and immunocytochemistry, the dilution range can vary from 1:100 to 1:500 depending on the target protein and cell type. Finally, for ELISA using a peptide, a dilution of 1:20000-1:40000 is advised to achieve optimal results. Keeping these dilution ratios in mind will help ensure accurate and reproducible experimental outcomes.
Immunogen	A synthesized peptide derived from human Sin3B.
MW	130kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the antiserum underwent purification through peptide affinity chromatography. To ensure high-quality results, this process was carefully carried out to produce a pure and effective antiserum.
Clonality	Polyclonal Antibody
Stability & Storage	It is recommended to keep the product at a temperature of -20 °C for a duration of one year. It is important to prevent the product from undergoing repeated freeze and thaw cycles.
Storage buffer	This product contains Rabbit IgG diluted in phosphate buffered saline with a pH of 7.4, combined with 150mM NaCl, 0.02% sodium azide, and 50% glycerol. For storage purposes, it is recommended to store this product at a temperature of -20 °C, where it will remain stable for up to 12 months from the date of receipt.
Target
Background	Acts as a transcriptional repressor. Interacts with MXI1 to repress MYC responsive genes and antagonize MYC oncogenic activities. Interacts with MAD-MAX heterodimers by binding to MAD. The heterodimer then represses transcription by tethering SIN3B to DNA. Also forms a complex with FOXK1 which represses transcription. With FOXK1, regulates cell cycle progression probably by repressing cell cycle inhibitor genes expression.
Posttranslational modification	Ubiquitinated by RNF220 that leads to proteasomal degradation.
Celluar localization	Nucleus;
UniPort	O75182