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Rabbit Anti-SELPLG Polyclonal Antibody#abs136056

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Description

Catalog-specification	Delivery time	USD price
abs136056-100ug	1-2 Weeks	301.0
abs136056-50ug	1-2 Weeks	201.0

Overview
catalog	abs136056
Description	This particular gene codes for a glycoprotein that acts as a high affinity counter-receptor for selectin cell adhesion molecules found on myeloid cells and activated T lymphocytes. It plays a crucial role in the movement of white blood cells during inflammation by attaching them to activated platelets or endothelial cells that express selectins. Two essential modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, are necessary for this protein to exhibit its strong binding activity. Abnormal expression of this gene and variations in its structure are linked to malfunctions in both the innate and adaptive immune responses. Multiple transcript variants are produced through alternative splicing of this gene.
Other names	The CD162 antigen, also known as CLA or cutaneous lymphocyte associated associated antigen, is a crucial molecule involved in the adhesion of leukocytes to endothelium during inflammation. P-selectin glycoprotein ligand 1 (PSGL-1) is the precursor of CD162 and serves as a high-affinity ligand for P-selectin, which is expressed by activated endothelial cells and platelets. PSGL-1 is also involved in the homing of T cells to lymphoid organs and leukocyte extravasation into tissues. Selectin P ligand is another name for PSGL-1, and the gene encoding this protein is SELPLG.
Source	Rabbit
Specificity	Total SELPLG levels can be accurately recognized by SELPLG Antibody, which specifically detects endogenous SELPLG quantity.
Species Reactivity	Human;Rat
Predictive reaction species	Horse
Antigen	SELPLG
Application	IHC 1:50-1:200, WB 1:500-1:2000, and ELISA(peptide) 1:20000-1:40000 are the recommended dilutions for these assays. The dilutions mentioned represent the ratios of antibody to sample or peptide.
Immunogen	A synthesized peptide derived from human SELPLG.
MW	120kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.
Tissue specificity	Expressed on neutrophils, monocytes and most lymphocytes.
Posttranslational modification	Displays complex, core-2, sialylated and fucosylated O-linked oligosaccharides, at least some of which appear to contain poly-N-acetyllactosamine with varying degrees of substitution. Mainly disialylated or neutral forms of the core-2 tetrasaccharide, Galbeta1-->4GlcNAcbeta1-->6(Galbeta1-->3)GalNAcOH. The GlcN:GalN ratio is approximately 2:1 and the Man:Fuc ratio 3:5. Contains about 14% fucose with alpha-1,3 linkage present in two forms: One species is a disialylated, monofucosylated glycan, and the other, a monosialylated, trifucosylated glycan with a polylactosamine backbone. The fucosylated forms carry the Lewis antigen and are important for interaction with selectins and for functioning in leukocyte rolling. The modification containing the sialyl Lewis X glycan is on Thr-57. No sulfated O-glycans. Some N-glycosylation.Sulfation, in conjunction with the SLe(x)-containing glycan, is necessary for P- and L-selectin binding. High affinity P-selectin binding has a preferred requirement for the isomer sulfated on both Tyr-48 and Tyr-51, whereas L-selectin binding requires predominantly sulfation on Tyr-51 with sulfation on Tyr-48 playing only a minor role. These sulfations play an important role in L- and P-selectin-mediated neutrophil recruitment, and leukocyte rolling.
Celluar localization	Plasma Membrane;
UniPort	Q14242