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Rabbit Anti-BTRC Polyclonal Antibody#abs136054

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Description

Catalog-specification	Delivery time	USD price
abs136054-100ug	1-2 Weeks	301.0
abs136054-50ug	1-2 Weeks	201.0

Overview
catalog	abs136054
Description	β-transducin repeat-containing protein, also known as β-TrCP or FBW1A, is a member of the F-box family of proteins, which possess a protein-protein mediating F-box domain initially discovered in cyclin F. F-box proteins play a crucial role in targeting specific phosphorylated proteins, known as phosphodegrons, to the SCF E3 ubiquitin ligase complex for ubiquitination. β-TrCP targets several vital proteins with diverse functions, including p53, H-Ras, Smad4, IκBα, β-catenin, and claspin, for ubiquitin-mediated degradation. Extensive research studies have established that blocking β-TrCP expression can be beneficial in prostate cancer treatment.
Other names	The Beta transducin repeat containing E3 ubiquitin protein ligase, also known as beta TrCP1, is a crucial component of the protein degradation mechanism in the human body. It is also commonly referred to as BETA-TRCP, betaTrCP, btrC, bTrCP, or bTrCP1. The importance of this protein lies in its ability to regulate the levels of various proteins present in the body, including IkappaBalpha, a protein that plays a crucial role in the NF-kappaB signaling pathway. Other aliases for Beta-TrCP include E3RSIkappaB, F box and WD repeat protein 1B, F box and WD repeats protein beta TrCP, F-box and WD repeats protein beta-TrCP, F-box/WD repeat protein 1A, F-box/WD repeat-containing protein 1A, FBW1A, FBW1A_HUMAN, FBXW1, FBXW1A, FWD1, HOS, mKIAA4123, pIkappaBalpha-E3 receptor subunit, and Slimb. Overall, Beta-TrCP is a critical player in maintaining the proper levels and functioning of proteins in the human body, thereby contributing to overall health and well-being.
Source	Rabbit
Specificity	The total BTRC levels are detected by the BTRC Antibody, which specifically targets the endogenous BTRC protein. I can generate content using a language model, but it may produce text that is significantly different from the original information.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog;Horse;Bovine
Antigen	BTRC
Application	For Western blotting, a dilution range of 1:500 to 1:2000 is recommended. Immunohistochemistry should be performed using a dilution range of 1:50 to 1:200. ELISA assays using peptides require a dilution range of 1:20000 to 1:40000 for optimal results. These dilution ranges have been established based on the original text and are appropriate for achieving accurate and reliable results.
Immunogen	A synthesized peptide derived from human BTRC.
MW	69kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins. SCF(BTRC) mediates the ubiquitination of CTNNB1 and participates in Wnt signaling. SCF(BTRC) mediates the ubiquitination of NFKBIA, NFKBIB and NFKBIE; the degradation frees the associated NFKB1 to translocate into the nucleus and to activate transcription. Ubiquitination of NFKBIA occurs at 'Lys-21' and 'Lys-22'. SCF(BTRC) mediates the ubiquitination of CEP68; this is required for centriole separation during mitosis (PubMed:25704143, PubMed:25503564). SCF(BTRC) mediates the ubiquitination of phosphorylated NFKB1/nuclear factor NF-kappa-B p105 subunit, ATF4, CDC25A, DLG1, FBXO5, PER1, SMAD3, SMAD4, SNAI1 and probably NFKB2. Has an essential role in the control of the clock-dependent transcription via degradation of phosphorylated PER1 and PER2. May be involved in ubiquitination and subsequent proteasomal degradation through a DBB1-CUL4 E3 ubiquitin-protein ligase. Required for activation of NFKB-mediated transcription by IL1B, MAP3K14, MAP3K1, IKBKB and TNF. Required for proteolytic processing of GLI3.
Tissue specificity	Expressed in epididymis (at protein level).
Celluar localization	Cytosol;Nucleus;
UniPort	Q9Y297