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Rabbit Anti-ROBO1 Polyclonal Antibody#abs139397

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Description

Catalog-specification	Delivery time	USD price
abs139397-100ug	1-2 Weeks	301.0
abs139397-50ug	1-2 Weeks	201.0

Overview
catalog	abs139397
Other names	FLJ21882, also known as Deleted in U twenty twenty, DUTT 1, H Robo 1, Robo 1, or SAX 3, is a roundabout homolog 1 (ROBO1) protein. It is encoded by the ROBO1_HUMAN gene and is involved in axon guidance. The precursor of Roundabout homolog1, also referred to as H-Robo-1 or Roundabout axon guidance receptor homolog 1, is represented by the gene symbol ROBO1_HUMAN. These names all pertain to the same protein, known as Roundabout 1, which has been extensively studied for its role in axon guidance.
Source	Rabbit
Specificity	The ROBO1 Antibody is capable of detecting total ROBO1 levels present within the body. The detection is able to capture the endogenous levels of the protein.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Pig;Rabbit;Sheep;Dog;Horse;Bovine;
Antigen	ROBO1
Application	WB 1:1000-3000, ELISA(peptide) 1:20000-1:40000
Immunogen	A synthesized peptide derived from human ROBO1.
MW	181 kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was employed for purifying the antiserum through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	To maintain the quality of the product, it is recommended to store it at a temperature of -20 °C for one year. It is important to avoid exposing the product to repeated freeze/thaw cycles, as this may negatively impact its efficacy. Therefore, it's best to take precautions and store the product in a cool, dry place to extend its shelf life.
Storage buffer	Storage conditions for Rabbit IgG are as follows: it should be stored at -20 °C in phosphate buffered saline with a pH of 7.4. The solution should contain 150mM NaCl, 0.02% sodium azide, and 50% glycerol. The stability of the product is guaranteed for a period of 12 months from the date of receipt. It is important to adhere to these storage instructions to ensure the integrity and functionality of the Rabbit IgG.
Target
Background	Receptor for SLIT1 and SLIT2 that mediates cellular responses to molecular guidance cues in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development (PubMed:10102268, PubMed:24560577). Interaction with the intracellular domain of FLRT3 mediates axon attraction towards cells expressing NTN1 (PubMed:24560577). In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex (By similarity). Plays a role in the regulation of cell migration via its interaction with MYO9B; inhibits MYO9B-mediated stimulation of RHOA GTPase activity, and thereby leads to increased levels of active, GTP-bound RHOA (PubMed:26529257). May be required for lung development (By similarity).
Tissue specificity	Widely expressed, with exception of kidney.
Posttranslational modification	Ubiquitinated. May be deubiquitinated by USP33.
Celluar localization	Plasma Membrane;
UniPort	Q9Y6N7