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Rabbit Anti-PSMB1 Polyclonal Antibody#abs135713

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Description

Catalog-specification	Delivery time	USD price
abs135713-100ug	1-2 Weeks	301.0
abs135713-50ug	1-2 Weeks	201.0

Overview
catalog	abs135713
Description	The proteasome, a multicatalytic proteinase complex, consists of a well-organized ring-shaped core structure called the 20S core. This core structure is made up of four rings comprising a total of 28 subunits, with each ring having a unique composition. Specifically, two rings are composed of 7 alpha subunits, while the other two rings consist of 7 beta subunits. Within eukaryotic cells, proteasomes are found abundantly and play a crucial role in peptide cleavage through a process that depends on ATP and ubiquitin. Unlike the lysosomal pathway, this process occurs outside the lysosomes. One important variant of the proteasome is the immunoproteasome, which is a modified form with specific functions. The immunoproteasome is primarily involved in the processing of class I MHC peptides. The gene we are discussing encodes a member of the proteasome B-type family, also referred to as the T1B family. As a 20S core beta subunit, it plays an essential role within the proteasome complex. Worth noting is the tight genetic linkage between this gene and the TBP (TATA-binding protein) gene in both human and mouse. Interestingly, these genes are transcribed in an opposite orientation in both species. Please note that while the generated content is based on the original text information, it has been rearranged to convey the same meaning in a different manner.
Other names	Proteasomes play a critical role in cellular proteolysis, regulating numerous cellular processes by degrading endogenous proteins such as misfolded or denatured proteins, and foreign proteins such as bacterial toxins. HC5, also known as Macropain subunit C5, is one of the subunits that forms the multicatalytic endopeptidase complex. It is responsible for the proteasome's beta-1 type activity. PSMB1 is another name for HC5 or Macropain subunit C5. It is a critical component of the proteasome gamma chain and is responsible for regulating the proteasome's overall function. It is involved in the breakdown of ubiquitinated proteins and helps to maintain protein homeostasis in the cell. Additionally, PSMB1 is involved in the degradation of damaged, mutated, or misfolded proteins, preventing them from accumulating and disrupting cellular function. KIAA1838 is yet another term used for HC5 or Macropain subunit C5. It has been identified as an essential protein for embryonic development and is localized to the proteasome. Recent studies have shown that KIAA1838 may have a role in inflammatory processes, as well as in the maintenance of protein homeostasis. Overall, the highly related terms used to refer to proteasome HC5 underscore the central role played by the proteasome in regulating multiple cellular processes. Understanding the complex interactions of the proteasome and its various subunits such as HC5 will be essential in developing novel therapeutic strategies for cancer, autoimmune diseases, and other proteasome-related disorders.
Source	Rabbit
Specificity	The PSMB1 Antibody is capable of detecting total levels of PSMB1 that are present naturally within the body. This information can be useful for a variety of research purposes, as it allows for a better understanding of how this protein functions and interacts within cells and tissues. By accurately detecting PSMB1 levels, researchers can gain valuable insights into a wide range of biological processes and systems.
Species Reactivity	Human;Mouse;Rat
Antigen	PSMB1
Application	We recommend using dilutions of WB 1:500-1:2000, IHC 1:50-1:200, and ELISA(peptide) 1:20000-1:40000 for optimal results. It is important to note that these dilutions may vary depending on the specific experiment, and adjustments may be necessary. Ensuring accurate dilution is key to obtaining reliable and reproducible results in these assays. As always, it is important to carefully follow the manufacturer's instructions and consult with experts in the field if you have any questions.
Immunogen	A synthesized peptide derived from human PSMB1.
MW	26kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
Celluar localization	Cytosol;Extracellular region or secreted;Nucleus;
UniPort	P20618