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Rabbit Anti-PSEN1 Polyclonal Antibody#abs136430

Description

Catalog-specification	Delivery time	USD price
abs136430-100ug	1-2 Weeks	301.0
abs136430-50ug	1-2 Weeks	201.0

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Overview
catalog	abs136430
Description	Presenilin 1 and presenilin 2, members of the presenilin family, are integral membrane proteins. Their genetic mutation has been implicated in the early onset of Alzheimer's disease, likely due to the role of presenilin in the processing of amyloid-β protein through its γ-secretase activity (1,2). In their natural state, presenilin forms a heterodimeric complex comprising of endoproteolytically processed amino-terminal (34 kDa) and carboxy-terminal (~20, 22, 23 kDa) fragments (CTF) (2,3). Anomalies in presenilin structure and function are thought to contribute to the pathogenesis of Alzheimer's disease by disrupting the normal processing of amyloid-β protein.
Other names	One possible rephrased content based on the original text information is: Homo Sapiens Clone CC44 Senilin 1, also known as AD3, Ad3h, FAD, Presenilin-1 CTF12, Protein S182, PS 1, PS-1, PS1-CTF12, PSEN1, PSNL1, or S182, is a crucial protein involved in the pathogenesis of Alzheimer's disease. It is a transmembrane protein that forms the catalytic core of gamma-secretase, an enzyme complex involved in the proteolysis of multiple substrates, including the amyloid precursor protein that generates the amyloid-beta peptide, a major component of the amyloid plaques seen in Alzheimer's disease. Mutations in the PSEN1 gene encoding Senilin 1 are associated with early-onset familial Alzheimer's disease, indicating the importance of this protein in the pathogenesis of the disease. Understanding the function and regulation of Senilin 1 and gamma-secretase complex may provide insights into the development of therapeutics for Alzheimer's disease.
Source	Rabbit
Specificity	The PSEN1 Antibody can detect the total amount of endogenous PSEN1 present in the sample. The antibody is effective in identifying all forms of PSEN1.
Species Reactivity	Human;Mouse;Rat
Antigen	PSEN1
Application	The recommended dilutions for Western blot (WB) range from 1:500 to 1:2000. For immunohistochemistry (IHC), the dilutions vary from 1:50 to 1:200. In the case of immunofluorescence or immunocytochemistry (IF/ICC), dilutions of 1:100 to 1:500 are suggested. For ELISA using peptide antigens, the dilutions are typically between 1:20000 and 1:40000. It is important to adhere to these dilution recommendations to obtain accurate and reliable results.
Immunogen	A synthesized peptide derived from human PSEN1.
MW	53kDa
Properties
Concentration	1mg/ml
purification	Purification of the antiserum was carried out utilizing SulfoLink™ Coupling Resin through the process of peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein) (PubMed:15274632, PubMed:10545183, PubMed:10593990, PubMed:10206644, PubMed:10899933, PubMed:10811883, PubMed:12679784, PubMed:12740439, PubMed:25043039, PubMed:26280335). Requires the presence of the other members of the gamma-secretase complex for protease activity (PubMed:15274632, PubMed:25043039, PubMed:26280335). Plays a role in Notch and Wnt signaling cascades and regulation of downstream processes via its role in processing key regulatory proteins, and by regulating cytosolic CTNNB1 levels (PubMed:9738936, PubMed:10593990, PubMed:10899933, PubMed:10811883). Stimulates cell-cell adhesion via its interaction with CDH1; this stabilizes the complexes between CDH1 (E-cadherin) and its interaction partners CTNNB1 (beta-catenin), CTNND1 and JUP (gamma-catenin) (PubMed:11953314). Under conditions of apoptosis or calcium influx, cleaves CDH1 (PubMed:11953314). This promotes the disassembly of the complexes between CDH1 and CTNND1, JUP and CTNNB1, increases the pool of cytoplasmic CTNNB1, and thereby negatively regulates Wnt signaling (PubMed:9738936, PubMed:11953314). Required for normal embryonic brain and skeleton development, and for normal angiogenesis (By similarity).
Tissue specificity	Detected in azurophile granules in neutrophils and in platelet cytoplasmic granules (at protein level) (PubMed:11987239). Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes (PubMed:7596406, PubMed:8641442, PubMed:8574969).
Posttranslational modification	Heterogeneous proteolytic processing generates N-terminal (NTF) and C-terminal (CTF) fragments of approximately 35 and 20 kDa, respectively. During apoptosis, the C-terminal fragment (CTF) is further cleaved by caspase-3 to produce the fragment, PS1-CTF12.After endoproteolysis, the C-terminal fragment (CTF) is phosphorylated on serine residues by PKA and/or PKC. Phosphorylation on Ser-346 inhibits endoproteolysis.
Celluar localization	Cytoskeleton;Endoplasmic reticulum;Golgi apparatus;Lysosome;Mitochondrion;Nucleus;Plasma Membrane;
UniPort	P49768