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Rabbit Anti-PROC Polyclonal Antibody#abs135932

Description

Catalog-specification	Delivery time	USD price
abs135932-100ug	1-2 Weeks	301.0
abs135932-50ug	1-2 Weeks	201.0

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Overview
catalog	abs135932
Description	The protein product of this gene is an important plasma glycoprotein that relies on vitamin K for its activation. It undergoes cleavage by the thrombin-thrombomodulin complex to achieve its activated form. Once activated, it possesses a serine protease domain and plays a crucial role in breaking down the activated forms of coagulation factors V and VIII. Genetic mutations within this gene have been linked to various disorders such as thrombophilia resulting from protein C deficiency, neonatal purpura fulminans, and recurring venous thrombosis. This information has been sourced from RefSeq, a comprehensive database (December 2009).
Other names	Protein C is a crucial protein involved in the regulation of blood coagulation. Also known as activation peptide, anticoagulant protein C, or autoprothrombin IIA, this protein plays a critical role in the inactivation of coagulation factors Va and VIIIa. It is encoded by the PROC gene and is an EC 3.4.21.69 enzyme. Protein C is synthesized as an inactive precursor molecule called proC. Vitamin K is required for its activation, which occurs through the action of the enzyme thrombin. The activated protein C (APC) then acts as an anticoagulant, inhibiting the clotting cascade by proteolytically inactivating factors Va and VIIIa. Inherited deficiencies in protein C can lead to venous thrombosis, while acquired deficiencies can occur in conditions such as liver disease or sepsis. Research on the role of protein C in various medical conditions is ongoing, with potential implications for the prevention and treatment of thrombotic diseases.
Source	Rabbit
Specificity	Endogenous levels of total PROC can be detected using the PROC Antibody.
Species Reactivity	Human;Mouse;Rat
Antigen	PROC
Application	The recommended dilutions for the western blot assay range from 1:500 to 1:2000, while for the immunohistochemistry assay, the suggested dilutions are within the range of 1:50 to 1:200. When attempting ELISA using a peptide, the optimal dilutions to use are between 1:20000 and 1:40000. By following these recommended dilution ranges, highly accurate and reliable results can be obtained for these assays. It is important to note the significance of optimal dilution when conducting any assays to avoid inaccurate data and unreliable results.
Immunogen	A synthesized peptide derived from human PROC.
MW	52kDa
Properties
Concentration	1mg/ml
purification	Using SulfoLink™ Coupling Resin, the purification of the antiserum was accomplished through peptide affinity chromatography.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed:25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed:25651845).
Tissue specificity	Plasma; synthesized in the liver.
Posttranslational modification	The vitamin K-dependent, enzymatic carboxylation of some Glu residues allows the modified protein to bind calcium.N- and O-glycosylated. Partial (70%) N-glycosylation of Asn-371 with an atypical N-X-C site produces a higher molecular weight form referred to as alpha. The lower molecular weight form, not N-glycosylated at Asn-371, is beta. O-glycosylated with core 1 or possibly core 8 glycans.The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.May be phosphorylated on a Ser or Thr in a region (AA 25-30) of the propeptide.
Celluar localization	Endoplasmic reticulum;Extracellular region or secreted;Golgi apparatus;
UniPort	P04070