Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-POLR2D Polyclonal Antibody#abs136184

Please note that the price provided is only for reference purpose. For more detailed pricing information, kindly contact our seller Vecent. It is important for us to emphasize that the details provided are based on the original text information. Therefore, we request you to get in touch with our...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136184-100ug	1-2 Weeks	301.0
abs136184-50ug	1-2 Weeks	201.0

Overview
catalog	abs136184
Description	The RNA polymerase II, responsible for synthesizing messenger RNA in eukaryotes, includes the fourth largest subunit encoded by this gene. In yeast, this particular subunit is associated with the polymerase during suboptimal growth conditions and may serve a role in protecting against stress. Interestingly, the transcript from this gene includes a ribosomal pseudogene sequence within its 3' untranslated region. This information was derived from RefSeq in July 2008.
Other names	The DNA directed RNA polymerase II 16 kDa polypeptide, also known as RPB4 or subunit D, plays a crucial role in transcriptional regulation. This protein is responsible for catalyzing the RNA synthesis from the DNA template during mRNA transcription. Its amino acid sequence is highly conserved across different species and it is required for the efficient processing of pre-mRNA. In humans, RPB4 is encoded by the polr2d gene. This subunit also interacts with other transcription factors and co-activators to control gene expression. Its importance in the regulation of gene expression has been demonstrated in numerous studies and mutations in the RPB4 gene have been linked with various disorders. Therefore, studying this protein and its interactions with other factors is crucial for a better understanding of gene regulation and its role in various biological processes.
Source	Rabbit
Specificity	The total POLR2D levels can be effectively detected by using the POLR2D Antibody to measure endogenous levels.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Rabbit;Pig;Dog
Antigen	POLR2D
Application	For Western blotting, the recommended dilutions are between 1:500 to 1:2000. When using immunohistochemistry, dilutions of 1:50 to 1:200 are suggested. For ELISA assays utilizing peptides, a dilution range of 1:20000 to 1:40000 should be used. It is important to follow these recommended dilutions in order to achieve accurate and reliable results in your experiments.
Immunogen	A synthesized peptide derived from human POLR2D.
MW	16kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB4 is part of a subcomplex with RPB7 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double-stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity).
Celluar localization	Cytosol;Nucleus;
UniPort	O15514