Phospho-Syntaxin 1A (Ser14) Rabbit Polyclonal Antibody#abs131088

abs131088-50ug

1-2 Weeks

201

abs131088-100ug

1-2 Weeks

301

Description

STX1A, a type IV membrane protein, is actively involved in the docking process of synaptic vesicles at presynaptic active zones. Its crucial role lies in facilitating the exocytosis of neurotransmitters. STX1A forms an integral part of the SNARE core complex, alongside SNAP25 and VAMP2. It is worth noting that there are three splice variant isoforms that have been identified in relation to STX1A.

Other names

STX1A, also known as Neuron-specific antigen HPC1 or Syntaxin 1A brain, is a protein that plays a crucial role in neuronal function. It is encoded by the HPC 1 gene and can be referred to as Neuron-specific antigen HPC-1 or P35-1.
The OTTHUMP00000174615, OTTHUMP00000174616, OTTHUMP00000174617, and OTTHUMP00000174618 are different transcript variants of STX1A. Each variant has specific functions and characteristics within the brain.
STX1A, or Syntaxin 1A, is a key component of the synaptic vesicle release machinery. It is involved in the exocytosis of neurotransmitters, allowing for the communication between neurons. This protein is localized in the presynaptic terminals of neurons, where it regulates vesicle docking and fusion with the plasma membrane.
Furthermore, STX1A is essential for synaptic transmission and the regulation of neurotransmitter release. It interacts with various proteins, including SNAP25 and VAMP2, to form the core complex responsible for synaptic vesicle fusion. This tight interaction ensures the efficient release of neurotransmitters and proper neuronal signaling.
In conclusion, STX1A, also known as Syntaxin 1A, is a neuron-specific antigen that plays a pivotal role in neuronal function. Its diverse transcript variants and interactions with other proteins make it an essential component of the synaptic vesicle release machinery.

Source

Rabbit

Specificity

The antibody Phospho-Syntaxin 1A (Ser14) is designed to specifically detect levels of Syntaxin 1A that have undergone phosphorylation at Serine 14. This antibody can accurately identify endogenous levels of Syntaxin 1A only when it has been modified through phosphorylation at this specific site.

Predictive reaction species

Sheep;Bovine;

Application

The recommended dilution for western blotting is 1:500 to 1:2000, while for immunofluorescence and immunocytochemistry the dilution range is 1:100 to 1:500. For ELISA using a peptide, the optimal dilution range is 1:20000 to 1:40000. It is important to adhere to these dilution ranges to ensure the best results for your experiment.

A peptide that has been synthesized is based on the human Syntaxin 1A protein and specifically targets the phosphorylation site of Serine 14. The peptide has been designed to closely resemble the original sequence in order to provide a highly specific and effective therapeutic option. By utilizing the natural properties of the human protein, this synthesized peptide is able to provide a targeted and precise treatment option for a variety of medical conditions. With its unparalleled ability to mimic the natural biology of the human body, this peptide represents a significant step forward in our ability to develop effective and safe therapies for a wide range of diseases and disorders.

Properties

1mg/ml

Purification

Through a process of affinity purification using sequential chromatography on phospho- and non-phospho-peptide affinity columns, the antibody was obtained from purified rabbit serum. This has resulted in a highly specific and pure antibody, ideally suited for use in a wide range of applications. The method used ensures that the antibody has the highest possible affinity for the specific target protein, while minimizing non-specific binding to other molecules. Overall, this process has produced an antibody of exceptional quality and purity, with proven efficacy in a variety of research settings.

Clonality

Polyclonal Antibody

To ensure proper storage of the product, it is recommended to keep it at a temperature of -20 °C for a period of one year. It is important to avoid subjecting the product to repeated cycles of freezing and thawing, as this could have a detrimental effect on its quality. Therefore, it is advised to store the product in conditions that are optimal for its longevity and effectiveness.

Storage buffer

This rabbit IgG is suspended in a solution of phosphate buffered saline at a pH of 7.4, containing 150mM NaCl, 0.02% sodium azide, and 50% glycerol. To maintain its stability, it should be stored at -20°C and will remain stable for up to 12 months from the date of receipt. It is important to handle this product with care and adhere to proper storage protocols to ensure its longevity and efficacy. This IgG is an important tool for biological research and can be used in a variety of applications, making it an essential component of many laboratory settings.

Target

Background

It has been suggested that this protein plays an important role in the secretion of hormones and neurotransmitters. Additionally, it may be involved in the process of docking synaptic vesicles at the active zones of presynaptic sites. Its function could extend to playing a role in regulating the acrosomal reaction in sperm through the regulation of calcium levels.

Tissue specificity

Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.

Celluar localization

Cytoskeleton;Cytosol;Extracellular region or secreted;Nucleus;Plasma Membrane;

Q16623