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Rabbit Anti-PCK1 Polyclonal Antibody#abs136290

Please note that the price provided is only for reference. For detailed pricing information, please contact our seller Vecent. It's important to connect with Vecent to get accurate pricing information. Thank you. Western blot analysis of extracts of variouscell lines, using PCK1 antibody. This...

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Description

Catalog-specification	Delivery time	USD price
abs136290-100ug	1-2 Weeks	301.0
abs136290-50ug	1-2 Weeks	201.0

Overview
catalog	abs136290
Description	PCK1, also known as PEPCK1, is part of the phosphoenolpyruvate carboxykinase [GTP] family, which plays a crucial role in the regulation of gluconeogenesis. Its main function is to catalyze the conversion of oxaloacetate into phosphoenolpyruvate while releasing carbon dioxide and GDP. In eukaryotes, this enzyme has two isozymes - a cytoplasmic one and a mitochondrial one. Notably, defects in PCK1 are associated with cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD). This antibody specifically reacts with PCK1 and is widely used for research purposes.
Other names	Cytosolic [GTP] is an important molecule involved in the regulation of many biochemical processes in the cell. One of the key enzymes that utilizes GTP as a substrate is Phosphoenolpyruvate carboxykinase 1 (PCK1). PCK1 catalyzes the conversion of phosphoenolpyruvate (PEP) to oxaloacetate (OAA) in the cytosol, thereby playing a critical role in gluconeogenesis, the process by which the body produces glucose from non-carbohydrate sources. The human PCK1 gene encodes for the PEP carboxykinase isoform known as PEPCK-C or PCKGC_HUMAN. This enzyme is also referred to as PCK1, PEPCK1, PEPCKC, or simply PEP carboxykinase. It is responsible for maintaining glucose homeostasis by regulating the levels of glucose in the blood. Phosphoenolpyruvate carboxykinase is an essential enzyme in the metabolic pathways of animals and plants. It is also involved in the biosynthesis of amino acids, nucleotides, and fatty acids. There are two main forms of this enzyme - the cytosolic [GTP] form, which is found in the cytosol of cells, and the mitochondrial form which is found in the mitochondria. Phosphoenolpyruvate carboxylase is another enzyme that is involved in the conversion of PEP to OAA. This enzyme is found in plants and some bacteria and is important in the process of photosynthesis. It catalyzes the formation of OAA from PEP and carbon dioxide (CO2), which is then used to produce glucose. Overall, the enzymes PCK1 and phosphoenolpyruvate carboxylase play important roles in regulating glucose homeostasis and metabolic pathways in the body, making them key targets for drug development in the treatment of metabolic disorders such as diabetes.
Source	Rabbit
Specificity	The PCK1 Antibody is capable of identifying the overall levels of endogenous PCK1 protein. This indicates that the antibody has the ability to detect all forms of PCK1 present within the sample being analyzed. By detecting the total PCK1 content, this antibody enables researchers to gain a better understanding of the protein's role in various cellular processes.
Species Reactivity	Human;Mouse;Rat
Antigen	PCK1
Application	The dilutions for WB range from 1:500 to 1:2000, for IHC they range from 1:50 to 1:200, and for ELISA (peptide) they range from 1:20000 to 1:40000. It is important to note that the dilutions mentioned above are based on the original information provided and should not be rearranged in the generated content.
Immunogen	A synthetic peptideof human PCK1.
MW	69kDa
Properties
Concentration	1mg/ml
purification	SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography in order to purify the antiserum. This enabled the efficient removal of impurities and resulted in a highly pure and effective antiserum product.
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
Tissue specificity	Major sites of expression are liver, kidney and adipocytes.
Posttranslational modification	Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2.Ubiquitination by UBR5 leads to proteasomal degradation.
Celluar localization	Cytosol;Extracellular region or secreted;
UniPort	P35558