Rabbit Anti-p55CDC Polyclonal Antibody#abs140621
Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent. This product is for research use only, not for use in diagnostic prodecures or in human.
Description
| Catalog-specification | Delivery time | USD price |
abs140621-100ug | 1-2 Weeks | 301.0 |
abs140621-50ug | 1-2 Weeks | 201.0 |
Please note that the price mentioned above is only for your reference. For detailed pricing information, please get in touch with our seller, Vecent.
| Overview | |
catalog | abs140621 |
| Other names | CDC20, also known as cell division cycle 20 homolog, plays a crucial role in the regulation of cell division. It is a protein found in humans and homologous to the CDC20 protein in the yeast Saccharomyces cerevisiae. The gene encoding CDC20 is referred to as CDC20A. In addition to its involvement in cell division, CDC20 is also known by other names such as fizzy, p55CDC, and MGC102824. Its function is to control the progression of cells through the various stages of cell division. By rearranging the information provided, we can highlight the significance and various names associated with CDC20: it is an essential protein involved in cell division regulation in humans, known as CDC20 or cell division cycle 20 homolog (CDC20_HUMAN). It is also referred to as CDC20A, cell division cycle protein 20 homolog, fizzy, p55CDC, or MGC102824. |
| Source | Rabbit |
| Specificity | The p55CDC Antibody has the capability to detect the overall amounts of p55CDC present in the body. This detection occurs at the level of endogenous production, enabling an accurate assessment of the total p55CDC concentration. Rearranging the content above, we can present this information in a slightly different way, while maintaining the same fundamental meaning. |
| Species Reactivity | Human;Mouse;Rat |
| Antigen | p55CDC |
| Application | For Western blotting, the recommended dilution range is 1:500 to 1:1000. In cell imaging techniques such as immunofluorescence/immunocytochemistry, a dilution of 1:100 to 1:500 is suggested. When performing ELISA with a peptide, it is advisable to use a dilution range of 1:20000 to 1:40000. These dilution ranges have been established based on the original text information to achieve optimal results for each respective technique. |
| Immunogen | A synthesized peptide. |
| MW | 50 KD |
| Properties | |
Concentration | 1mg/ml |
| purification | The SulfoLink™ Coupling Resin was utilized for peptide affinity chromatography to purify the antiserum. |
| Clonality | Polyclonal Antibody |
| Stability & Storage | To ensure preservation, it is advisable to store the item at a temperature of -20 °C for a duration of one year. It is crucial to prevent subjecting it to multiple cycles of freezing and thawing. |
| Storage buffer | This product is a solution of Rabbit IgG in a phosphate buffered saline with a pH of 7.4, containing 150 millimolar NaCl, 0.02 percent sodium azide and 50 percent glycerol. It should be stored at a temperature of -20 degrees Celsius and is stable for up to 12 months from the date of receipt. |
Target | |
Background | Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. |
| Tissue specificity | Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition. |
| Posttranslational modification | Acetylated. Deacetylated at Lys-66 by SIRT2; deacetylation enhances the interaction of CDC20 with CDC27, leading to activation of anaphase promoting complex/cyclosome (APC/C).Phosphorylated during mitosis, probably by maturation promoting factor (MPF). Phosphorylated by BUB1 at Ser-41; Ser-72; Ser-92; Ser-153; Thr-157 and Ser-161. Phosphorylated by NEK2.Dephosphorylated by CTDP1.Ubiquitinated and degraded by the proteasome during spindle assembly checkpoint. Deubiquitinated by USP44, leading to stabilize the MAD2L1-CDC20-APC/C ternary complex, thereby preventing premature activation of the APC/C. Ubiquitinated at Lys-490 during prometaphase. Ubiquitination at Lys-485 and Lys-490 has no effect on its ability to bind the APC/C complex. |
| Celluar localization | Cytoskeleton;Cytosol;Nucleus; |
| UniPort | Q12834 |
This product is for research use only, not for use in diagnostic prodecures or in human.
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