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Rabbit Anti-OTX2 Polyclonal Antibody#abs136872

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Description

Catalog-specification	Delivery time	USD price
abs136872-100ug	1-2 Weeks	301.0
abs136872-50ug	1-2 Weeks	201.0

Dear customers,
Please note that the mentioned price is only for your reference. For the detailed price, we kindly request you to get in touch with our seller, Vecent. They will provide you with all the necessary information regarding pricing. We value your interest and look forward to assisting you further.
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Overview
catalog	abs136872
Description	The transcription factor encoded by this gene belongs to the bicoid subfamily and contains a homeodomain. It has significant involvement in the development of the brain, craniofacial features, and sensory organs. Additionally, it plays a crucial role in the regulation of mitosis, specifically in the proliferation and differentiation of dopaminergic neuronal progenitor cells. Defects or mutations in this gene contribute to the development of both syndromic microphthalmia 5 (MCOPS5) and combined pituitary hormone deficiency 6 (CPHD6). Interestingly, there is some suspicion regarding the oncogenic potential of this gene in medulloblastoma. Alternative splicing of this gene leads to the production of various transcript variants, resulting in distinct protein isoforms. Furthermore, pseudogenes of this gene can be found on chromosomes two and nine.
Other names	OTX2, also known as Orthodenticle homolog 2, is a homeobox protein that plays a crucial role in embryonic development and organ formation. Identified as MCOPS 5 or MGC45000, this protein is highly conserved across different species. Its expression is vital for the development of the brain, eyes, and other sensory organs. OTX2 acts as a transcription factor, regulating the expression of various target genes involved in cell differentiation and tissue development. Its function is well-studied in Drosophila, where it is known as Orthodenticle homolog 2. However, its significance in human biology has also been extensively investigated. Notably, mutations in the OTX2 gene have been associated with various developmental disorders, including microphthalmia and other ocular abnormalities. OTX2 is a fascinating molecule that continues to intrigue researchers due to its essential role in shaping the intricate complexities of embryogenesis.
Source	Rabbit
Specificity	The OTX2 Antibody is designed to detect total endogenous levels of OTX2. This means that the antibody is capable of identifying all forms of OTX2 that are present naturally within the cells being analyzed. By targeting the total amount of OTX2, the antibody provides researchers with a comprehensive understanding of the protein's expression levels and distribution within the cells of interest. Overall, the OTX2 Antibody is a valuable tool for investigating the role of OTX2 in various biological processes.
Species Reactivity	Human;Mouse;Rat
Predictive reaction species	Xenopus;Rabbit;Pig;Dog;Sheep;Bovine;Horse
Antigen	OTX2
Application	The recommended dilutions for using this antibody in different applications are as follows: for Western blotting, a dilution range of 1:500 to 1:2000 is recommended; for immunohistochemistry, the suggested dilution range is 1:50 to 1:200; for ELISA with peptide antigens, a dilution range of 1:20000 to 1:40000 is advised. By following these guidelines, you can ensure optimal performance of the antibody in your experiments.
Immunogen	A synthesized peptide derived from human OTX2.
MW	31kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Transcription factor probably involved in the development of the brain and the sense organs. Can bind to the bicoid/BCD target sequence (BTS): 5'-TCTAATCCC-3'.
Tissue specificity	At Carnegie stage (CS) 14, widely expressed throughout the telencephalon and mesencephalon, with a sharp cutoff at the midbrain-hindbrain boundary. At CS16, found in the lamina terminalis and the floor of the telencephalon. At CS16-CS19, in the developing eye, strongly expressed in the retinal pigment epithelium layer and more weakly in the neural retina, not expressed in the optic nerve itself (at protein level). Retinal expression peaks between CS19 and CS21 and decline in older fetuses. At CS22, detected in the choroid plexus, the dorsal thalamus, and the roof of the mesencephalon. In the developing nasal structures, expressed in the olfactory epithelium of the nasal pits at CS18.
Celluar localization	Nucleus;
UniPort	P32243