Home - Products - Antibodies - Primary Antibodies - Polyclonal Antibody - Details

Rabbit Anti-NR0B2 Polyclonal Antibody#abs136168

Please note that the price mentioned here is only for your reference. For further details regarding the price, kindly get in touch with our seller Vecent. We request you to kindly rearrange the given information and generate a similar content that is based on the original text. Please ensure...

Send Inquiry

Description

Catalog-specification	Delivery time	USD price
abs136168-100ug	1-2 Weeks	301.0
abs136168-50ug	1-2 Weeks	201.0

Overview
catalog	abs136168
Description	This particular gene encodes for a rather unusual orphan receptor that contains a putative ligand-binding domain, but does not have a typical DNA-binding domain. Its product belongs to the nuclear hormone receptor family, which is a group of transcription factors that are regulated by small hydrophobic hormones. Some of these receptors do not have any known ligands, so they are called orphan nuclear receptors. Recent studies have shown that this particular protein interacts with retinoid and thyroid hormone receptors, which helps inhibit their ligand-dependent transcriptional activation. It also interacts with estrogen receptors, leading to a decrease in their function. Furthermore, research suggests that this protein represses nuclear hormone receptor-mediated transactivation through two separate mechanisms. Firstly, it competes with coactivators, which helps prevent the activation of hormone receptors. Secondly, the protein's transcriptional repressor function directly affects the transcriptional activation process. These findings give us a better understanding of the intricate regulatory pathways that govern hormone receptor activity.
Other names	Nr0b2a, also known as nuclear receptor subfamily 0 group B member 2a or small heterodimer partner (SHP), is an orphan nuclear receptor. It plays a crucial role in regulating various biological processes. SHP acts as a transcriptional repressor by interacting with other nuclear receptors and inhibiting their activity. It is involved in the regulation of glucose and lipid metabolism, bile acid synthesis, and liver function. SHP is expressed in various tissues, including the liver, adipose tissue, and intestine. Its dysregulation has been implicated in several diseases, including obesity, diabetes, and liver cancer. Understanding the molecular mechanisms underlying SHP function may provide new insights into the development of therapeutic strategies for these diseases.
Source	Rabbit
Specificity	The NR0B2 Antibody is capable of identifying total NR0B2 levels that occur naturally in the body. To create analogous content, the text could be rearranged to read: The endogenous levels of total NR0B2 can be detected by the NR0B2 Antibody.
Species Reactivity	Human;Mouse
Predictive reaction species	Rabbit;Pig;Dog;Horse;
Antigen	NR0B2
Application	The recommended dilutions for using this antibody are: for Western blotting, use a dilution of 1:500 to 1:2000; for immunohistochemistry, use a dilution of 1:50 to 1:200; for immunofluorescence or immunocytochemistry, use a dilution of 1:100 to 1:500; for ELISA using the peptide, use a dilution of 1:20000 to 1:40000. These recommended dilutions have been established based on the results of testing and experimentation, and it is important to follow them in order to obtain optimal results. It is also important to note that the actual optimal dilution may vary depending on the sample type and other experimental conditions.
Immunogen	A synthesized peptide derived from human NR0B2.
MW	28kDa
Properties
Concentration	1mg/ml
purification	The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .
Clonality	Polyclonal Antibody
Stability & Storage	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles
Storage buffer	Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Target
Background	Acts as a transcriptional regulator. Acts as a negative regulator of receptor-dependent signaling pathways. Specifically inhibits transactivation of the nuclear receptor with whom it interacts. Inhibits transcriptional activity of NEUROD1 on E-box-containing promoter by interfering with the coactivation function of the p300/CBP-mediated transcription complex for NEUROD1.
Tissue specificity	Liver. Low levels of expression were detected in heart and pancreas.
Posttranslational modification	Arginine methylation by PRMT5 enhances repression activity of metabolic genes in liver in response to bile acid signaling, by increasing interaction with cofactors.
Celluar localization	Nucleus;
UniPort	Q15466